In situ synthesis of biomolecule encapsulated gold-cross-linked poly(ethylene glycol) nanocomposite as biosensing platform: A model study
Date
2010Source Title
Bioelectrochemistry
Print ISSN
1878-562X
Publisher
Elsevier BV
Volume
79
Issue
2
Pages
211 - 217
Language
English
Type
ArticleItem Usage Stats
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Abstract
In situ synthesis of poly(ethylene glycol) (PEG) hydrogels containing gold nanoparticles(AuNPs) and glucose oxidase (GOx) enzyme by photo-induced electron transfer process was reported here and applied in electrochemical glucose biosensing as the model system. Newly designed bionanocomposite matrix by simple one-step fabrication offered a good contact between the active site of the enzyme and AuNPs inside the network that caused the promotion in the electron transfer properties that was evidenced by cyclic voltammetryas well as higher amperometric biosensing responses in comparing with response signals obtained from the matrix without AuNPs. As well as some parameters important in the optimization studies such as optimum pH, enzyme loading and AuNP amount, the analytical characteristics of the biosensor (AuNP/GOx) were examined by the monitoring of chronoamperometric response due to the oxygen consumption through the enzymatic reaction at − 0.7 V under optimized conditions at sodium acetate buffer (50 mM, pH 4.0) and the linear graph was obtained in the range of 0.1–1.0 mM glucose. The detection limit (LOD) of the biosensor was calculated as 0.06 mM by using the signal to noise ratio of 3. Moreover, the presence of AuNPs was visualized by TEM. Finally, the biosensor was applied for glucose analysis for some beverages and obtained data were compared with HPLC as the reference method to test the possible matrix effect due to the nature of the samples.
Keywords
BionanocompositeBiosensor
Gold nanoparticles
Hydrogel
Photopolymerization
Active site
Amperometric
Bionanocomposite
Biosensing
Biosensing platforms
Chronoamperometric response
Detection limits
Electron transfer
Enzymatic reaction
Enzyme loading
Glucose analysis
Gold nanoparticle
Gold Nanoparticles
In-situ synthesis
Linear graph
matrix
Matrix effects
Model study
Model system
Optimization studies
Optimized conditions
Optimum pH
Oxygen consumption
Photo-induced electron transfer
Reference method
Response signal
Sodium-acetate buffer
TEM
Beverages
Cyclic voltammetry
Electron transitions
Enzymes
Ethylene
Ethylene glycol
Glucose
Glucose oxidase
Glucose sensors
Gold
Hydrogels
Matrix algebra
Nanoparticles
Optimization
Oxygen
Photopolymerization
Polyethylene glycols
Polyethylene oxides
Signal to noise ratio
Sodium
Synthesis (chemical)
Biosensors
gold nanoparticle
macrogol
nanocomposite
oxygen
amperometry
article
biosensor
calculation
cross linking
cyclic potentiometry
encapsulation
enzyme active site
enzyme mechanism
high performance liquid chromatography
hydrogel
monitoring
oxygen consumption
particle size
pH
polymerization
signal noise ratio
synthesis
transmission electron microscopy
Beverages
Biosensing Techniques
Buffers
Chromatography, High Pressure Liquid
Electrochemistry
Electrodes
Glucose
Glucose Oxidase
Gold
Hydrogen-Ion Concentration
Limit of Detection
Metal Nanoparticles
Microscopy, Electron, Transmission
Nanocomposites
Oxidation-Reduction
Polyethylene Glycols
Sodium Acetate
Permalink
http://hdl.handle.net/11693/22196Published Version (Please cite this version)
http://dx.doi.org/10.1016/j.bioelechem.2010.05.001Collections
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