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      In situ synthesis of biomolecule encapsulated gold-cross-linked poly(ethylene glycol) nanocomposite as biosensing platform: A model study

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      Author(s)
      Odaci, D.
      Kahveci, M.U.
      Sahkulubey, E.L.
      Ozdemir, C.
      Uyar, Tamer
      Timur, S.
      Yagci Y.
      Date
      2010
      Source Title
      Bioelectrochemistry
      Print ISSN
      1878-562X
      Publisher
      Elsevier BV
      Volume
      79
      Issue
      2
      Pages
      211 - 217
      Language
      English
      Type
      Article
      Item Usage Stats
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      132
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      Abstract
      In situ synthesis of poly(ethylene glycol) (PEG) hydrogels containing gold nanoparticles(AuNPs) and glucose oxidase (GOx) enzyme by photo-induced electron transfer process was reported here and applied in electrochemical glucose biosensing as the model system. Newly designed bionanocomposite matrix by simple one-step fabrication offered a good contact between the active site of the enzyme and AuNPs inside the network that caused the promotion in the electron transfer properties that was evidenced by cyclic voltammetryas well as higher amperometric biosensing responses in comparing with response signals obtained from the matrix without AuNPs. As well as some parameters important in the optimization studies such as optimum pH, enzyme loading and AuNP amount, the analytical characteristics of the biosensor (AuNP/GOx) were examined by the monitoring of chronoamperometric response due to the oxygen consumption through the enzymatic reaction at − 0.7 V under optimized conditions at sodium acetate buffer (50 mM, pH 4.0) and the linear graph was obtained in the range of 0.1–1.0 mM glucose. The detection limit (LOD) of the biosensor was calculated as 0.06 mM by using the signal to noise ratio of 3. Moreover, the presence of AuNPs was visualized by TEM. Finally, the biosensor was applied for glucose analysis for some beverages and obtained data were compared with HPLC as the reference method to test the possible matrix effect due to the nature of the samples.
      Keywords
      Bionanocomposite
      Biosensor
      Gold nanoparticles
      Hydrogel
      Photopolymerization
      Active site
      Amperometric
      Bionanocomposite
      Biosensing
      Biosensing platforms
      Chronoamperometric response
      Detection limits
      Electron transfer
      Enzymatic reaction
      Enzyme loading
      Glucose analysis
      Gold nanoparticle
      Gold Nanoparticles
      In-situ synthesis
      Linear graph
      matrix
      Matrix effects
      Model study
      Model system
      Optimization studies
      Optimized conditions
      Optimum pH
      Oxygen consumption
      Photo-induced electron transfer
      Reference method
      Response signal
      Sodium-acetate buffer
      TEM
      Beverages
      Cyclic voltammetry
      Electron transitions
      Enzymes
      Ethylene
      Ethylene glycol
      Glucose
      Glucose oxidase
      Glucose sensors
      Gold
      Hydrogels
      Matrix algebra
      Nanoparticles
      Optimization
      Oxygen
      Photopolymerization
      Polyethylene glycols
      Polyethylene oxides
      Signal to noise ratio
      Sodium
      Synthesis (chemical)
      Biosensors
      gold nanoparticle
      macrogol
      nanocomposite
      oxygen
      amperometry
      article
      biosensor
      calculation
      cross linking
      cyclic potentiometry
      encapsulation
      enzyme active site
      enzyme mechanism
      high performance liquid chromatography
      hydrogel
      monitoring
      oxygen consumption
      particle size
      pH
      polymerization
      signal noise ratio
      synthesis
      transmission electron microscopy
      Beverages
      Biosensing Techniques
      Buffers
      Chromatography, High Pressure Liquid
      Electrochemistry
      Electrodes
      Glucose
      Glucose Oxidase
      Gold
      Hydrogen-Ion Concentration
      Limit of Detection
      Metal Nanoparticles
      Microscopy, Electron, Transmission
      Nanocomposites
      Oxidation-Reduction
      Polyethylene Glycols
      Sodium Acetate
      Permalink
      http://hdl.handle.net/11693/22196
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.bioelechem.2010.05.001
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