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      Age-related synapse loss in hippocampal CA3 is not reversed by caloric restriction

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      Author
      Adams, Michelle M.
      Donohue, H. S.
      Linville, M. C.
      Iversen, E. A.
      Newton, I. G.
      Bechtold, J. K. B.
      Date
      2010
      Source Title
      Synapses
      Print ISSN
      0306-4522
      Electronic ISSN
      1873-7544
      Publisher
      Pergamon Press
      Volume
      171
      Issue
      2
      Pages
      373 - 382
      Language
      English
      Type
      Article
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      Abstract
      Caloric restriction (CR) is a reduction of total caloric intake without a decrease in micronutrients or a disproportionate reduction of any one dietary component. While CR attenuates age-related cognitive deficits in tasks of hippocampal-dependent memory, the cellular mechanisms by which CR improves this cognitive decline are poorly understood. Previously, we have reported age-related decreases in key synaptic proteins in the CA3 region of the hippocampus that are stabilized by lifelong CR. In the present study, we examined possible age-related changes in the functional microcircuitry of the synapses in the stratum lacunosum-moleculare (SL-M) of the CA3 region of the hippocampus, and whether lifelong CR might prevent these age-related alterations. We used serial electron microscopy to reconstruct and classify SL-M synapses and their postsynaptic spines. We analyzed synapse number and size as well as spine surface area and volume in young (10 months) and old (29 months) ad libitum fed rats and in old rats that were calorically restricted from 4 months of age. We limited our analysis to SL-M because previous work demonstrated age-related decreases in synaptophysin confined to this specific layer and region of the hippocampus. The results revealed an age-related decrease in macular axo-spinous synapses that was not reversed by CR that occurred in the absence of changes in the size of synapses or spines. Thus, the benefits of CR for CA3 function and synaptic plasticity may involve other biological effects including the stabilization of synaptic proteins levels in the face of age-related synapse loss. © 2010 IBRO.
      Keywords
      Dietary restriction
      Electron microscopy
      Hippocampus
      Rat
      Serial reconstruction
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      http://hdl.handle.net/11693/22128
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.neuroscience.2010.09.022
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      • Aysel Sabuncu Brain Research Center (BAM) 183
      • Department of Psychology 157
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