Expression of CK-19 and CEA mRNA in peripheral blood of gastric cancer patients
Author
Kutun, S.
Celik, A.
Cem Kockar, M.
Erkorkmaz, U.
Eroǧlu, A.
Cetin, A.
Erkosar, B.
Yakicier, C.
Date
2010Source Title
Experimental Oncology
Print ISSN
18129269
Volume
32
Issue
4
Pages
263 - 268
Language
English
Type
ArticleItem Usage Stats
85
views
views
30
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downloads
Abstract
Aim: To investigate the clinical and pathological relevance of detection of circulating tumor cells (CTC) in the peripheral blood of gastric carcinoma patients before operation. Patients and Methods: Fifty patients with gastric adenocarcinoma were analysed prospectively. Patients were divided into two groups according to the extent of the tumor. Group I (unresectable) consisted of 22, and group II (resectable) consisted of 28 patients. Peripheral blood samples were collected pre-operatively from all 50 patients as well as from ten healthy controls and analyzed for carcinoembryonic antigen (CEA) and cytokeratin-19 (CK-19) messenger ribonucleic acids (mRNAs). Tumor localisation, stage, presence of signet cell formation, nodal metastases, serousal and lymphovascular invasion were recorded for all patients. Results: Expression of CK-19 was detected in 24 (48%), and CEA in 10 (20%) cases. Nine patients (40%) in group I and 15 (53.6%) in group II were positive for CK-19 expression. CEA expression was more frequent among group I patients (6 vs. 4 cases). There was no significant difference between the groups in the expression of CK-19 and CEA mRNA, tumor localisation, presence of signet formation, and presence and extent of nodal metastases. Patients with major vascular invasion (MVI) expressed significantly higher levels of CTC mRNA compared to those without MVI (p = 0.023 for CEA, and p = 0.009 for CK-19). The median 1 and 2-year survival was 9.5 and 10.5 months for group I, and 20 and 28.5 months for group II, respectively (p = 0.001). The mean survival was 6.7 months for patients with MVI, and 30.2 months for those without MVI (p = 0.0001). Conclusions: High levels of CTCs were observed in patients with MVI invasion, rather than other causes of unresectability. It can be suggested that expression of both CEA and CK-19 in the peripheral blood of gastric cancer patients are strong predictors of MVI and significantly worse survival rates. Copyright © Experimental Oncology, 2010.
Keywords
Carcinoembryonic antigenCytokeratin-19
Gastric carcinoma
Micrometastases
mRNA
Vascular invasion
carcinoembryonic antigen
cytokeratin 19
fluorouracil
folinic acid
messenger RNA
adult
aged
antigen expression
article
cancer invasion
cancer localization
cancer patient
cancer surgery
cancer survival
cell
controlled study
disease severity
female
human
human cell
human tissue
lymph node metastasis
lymph vessel
major clinical study
male
metastasis
multiple cycle treatment
predictor variable
prospective study
signet cell
stomach adenocarcinoma
stomach carcinoma
survival rate
survival time
tumor cell
tumor localization
Adenocarcinoma
Adult
Aged
Aged, 80 and over
Carcinoembryonic Antigen
Female
Humans
Kaplan-Meier Estimate
Keratin-19
Male
Middle Aged
Neoplastic Cells, Circulating
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Stomach Neoplasms
Tumor Markers, Biological
Permalink
http://hdl.handle.net/11693/22085Collections
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