Show simple item record

dc.contributor.authorCinar, B.en_US
dc.contributor.authorCollak F.K.en_US
dc.contributor.authorLopez, D.en_US
dc.contributor.authorAkgul, S.en_US
dc.contributor.authorMukhopadhyay, N.K.en_US
dc.contributor.authorKilicarslan, M.en_US
dc.contributor.authorGioeli, D.G.en_US
dc.contributor.authorFreeman, M.R.en_US
dc.date.accessioned2016-02-08T09:52:36Z
dc.date.available2016-02-08T09:52:36Z
dc.date.issued2011en_US
dc.identifier.issn0008-5472
dc.identifier.urihttp://hdl.handle.net/11693/21888
dc.description.abstractThe MST1 serine - threonine kinase, a component of the RASSF1-LATS tumor suppressor network, is involved in cell proliferation and apoptosis and has been implicated in cancer. However, the physiologic role of MST1 in prostate cancer (PCa) is not well understood. Here, we investigated the possibility of a biochemical and functional link between androgen receptor (AR) and MST1 signaling. We showed that MST1 forms a protein complex with AR and antagonizes AR transcriptional activity as shown by coimmunoprecipitation (co-IP), promoter reporter analysis, and molecular genetic methods. In vitro kinase and site-specific mutagenesis approaches indicate that MST1 is a potent AR kinase; however, the kinase activity of MST1 and its proapoptotic functions were shown not to be involved in inhibition of AR. MST1 was also found in AR - chromatin complexes, and enforced expression of MST1 reduced the binding of AR to a well-characterized, androgen-responsive region within the prostate-specific antigen promoter. MST1 suppressed PCa cell growth in vitro and tumor growth in mice. Because MST1 is also involved in regulating the AKT1 pathway, this kinase may be an important new link between androgenic and growth factor signaling and a novel therapeutic target in PCa. ©2011 AACR.en_US
dc.language.isoEnglishen_US
dc.source.titleCancer Researchen_US
dc.relation.isversionofhttp://dx.doi.org/10.1158/0008-5472.CAN-10-4532en_US
dc.subjectandrogen receptoren_US
dc.subjectprostate specific antigenen_US
dc.subjectprotein MST1en_US
dc.subjectprotein serine threonine kinaseen_US
dc.subjectunclassified drugen_US
dc.subjectanimal cellen_US
dc.subjectanimal experimenten_US
dc.subjectanimal modelen_US
dc.subjectarticleen_US
dc.subjectcancer inhibitionen_US
dc.subjectcontrolled studyen_US
dc.subjectenzyme activityen_US
dc.subjectgrowth inhibitionen_US
dc.subjecthormone bindingen_US
dc.subjecthormone inhibitionen_US
dc.subjecthormone protein complexen_US
dc.subjectimmunoprecipitationen_US
dc.subjectin vitro studyen_US
dc.subjectin vivo studyen_US
dc.subjectmaleen_US
dc.subjectmolecular geneticsen_US
dc.subjectmouseen_US
dc.subjectnonhumanen_US
dc.subjectpriority journalen_US
dc.subjectpromoter regionen_US
dc.subjectprostate canceren_US
dc.subjectsignal transductionen_US
dc.subjectsite directed mutagenesisen_US
dc.subjecttranscription initiationen_US
dc.subjectAndrogen Receptor Antagonistsen_US
dc.subjectAnimalsen_US
dc.subjectCaspasesen_US
dc.subjectCercopithecus aethiopsen_US
dc.subjectChromatinen_US
dc.subjectCOS Cellsen_US
dc.subjectHEK293 Cellsen_US
dc.subjectHumansen_US
dc.subjectMaleen_US
dc.subjectMiceen_US
dc.subjectPhosphorylationen_US
dc.subjectProstatic Neoplasmsen_US
dc.subjectProtein-Serine-Threonine Kinasesen_US
dc.subjectProto-Oncogene Proteins c-akten_US
dc.subjectReceptors, Androgenen_US
dc.subjectSignal Transductionen_US
dc.titleMST1 is a multifunctional caspase-independent inhibitor of androgenic signalingen_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.citation.spage4303en_US
dc.citation.epage4313en_US
dc.citation.volumeNumber71en_US
dc.citation.issueNumber12en_US
dc.identifier.doi10.1158/0008-5472.CAN-10-4532en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record