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      TIMP-2 gene transfer by positively charged PEG-lated monosized polycationic carrier to smooth muscle cells

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      Author
      Laçin, N.
      Utkan, G.
      Kutsal, T.
      Dedeoğlu, B. G.
      Yuluğ, I. G.
      Pişkin, E.
      Date
      2012
      Source Title
      Journal of Nanoparticle Research
      Print ISSN
      1388-0764
      Electronic ISSN
      1572-896X
      Publisher
      Springer Netherlands
      Volume
      14
      Issue
      2
      Pages
      1 - 9
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      Remodeling of the extracellular matrix resulting from increased secretion of metalloproteinase enzymes is implicated in restenosis following balloon angioplasty. Matrix metalloproteinases (MMPs) and the tissue inhibitors of metalloproteinases play an essential role in both normal and pathological extracellular matrix degradation. Tissue inhibitor of matrix metalloproteinase- 2 is the most extensively studied tissue inhibitor of metalloproteinases inmyocardial tissue in animalmodels and clinical examples of cardiac disease; therefore it is selected for this study. Gene transfer of tissue inhibitor of matrix metalloproteinase-2 may have a therapeutic potential by inhibition of matrix metalloproteinase activity. We have used PEG-lated nanoparticles poly(St/PEG-EEM/DMAPM) which were synthesized previously in our laboratory. The nanoparticles, with an average size of 77.6 ± 2.05 nm with a zeta potential of +64. 4 ± 1.14 mVand 201.9 ± 1.83 nmwith +54.2 ± 0.77 mV were used in the transfection studies. Zeta Potential values and size of polyplex were appropriate for an effective transfection. TIMP-2 expression was detected by western blotting. Increased protein level in smoothmuscle cells according to non-transfected smooth muscle cells confirms the successful delivery and expression of the tissue inhibitor of matrix metalloproteinase- 2 gene with the non-viral vector transfection approach. © Springer Science+Business Media B.V. 2012.
      Keywords
      Gene therapy
      Nanomedicine
      Nanoparticles
      SMC
      TIMP
      Permalink
      http://hdl.handle.net/11693/21607
      Published Version (Please cite this version)
      http://dx.doi.org/10.1007/s11051-011-0694-3
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