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dc.contributor.authorLigons, D. L.en_US
dc.contributor.authorTuncer, C.en_US
dc.contributor.authorLinowes, B. A.en_US
dc.contributor.authorAkcay, I. M.en_US
dc.contributor.authorKurtulus, S.en_US
dc.contributor.authorDeniz, E.en_US
dc.contributor.authorArslan, B. A.en_US
dc.contributor.authorCevik, S. I.en_US
dc.contributor.authorKeller, H. R.en_US
dc.contributor.authorLuckey, M. A.en_US
dc.contributor.authorFeigenbaum, L.en_US
dc.contributor.authorMöröy, T.en_US
dc.contributor.authorErsahin, T.en_US
dc.contributor.authorAtalay, R.en_US
dc.contributor.authorErman, B.en_US
dc.contributor.authorPark, J. H.en_US
dc.date.accessioned2016-02-08T09:44:32Z
dc.date.available2016-02-08T09:44:32Z
dc.date.issued2012en_US
dc.identifier.issn0021-9258
dc.identifier.urihttp://hdl.handle.net/11693/21307
dc.description.abstractInterleukin-7 receptor α (IL-7Rα) is essential for T cell survival and differentiation. Glucocorticoids are potent enhancers of IL-7Rα expression with diverse roles in T cell biology. Here we identify the transcriptional repressor, growth factor independent-1 (Gfi1), as a novel intermediary in glucocorticoid-induced IL-7Rα up-regulation. We found Gfi1 to be a major inhibitory target of dexamethasone by microarray expression profiling of 3B4.15 T-hybridoma cells. Concordantly, retroviral transduction of Gfi1 significantly blunted IL-7Rα up-regulation by dexamethasone. To further assess the role of Gfi1 in vivo, we generated bacterial artificial chromosome (BAC) transgenic mice, in which a modified Il7r locus expresses GFP to report Il7r gene transcription. By introducing this BAC reporter transgene into either Gfi1-deficient or Gfi1-transgenic mice, we document in vivo that IL-7Rα transcription is up-regulated in the absence of Gfi1 and down-regulated when Gfi1 is overexpressed. Strikingly, the in vivo regulatory role of Gfi1 was specific for CD8+, and not CD4+ T cells or immature thymocytes. These results identify Gfi1 as a specific transcriptional repressor of the Il7r gene in CD8 T lymphocytes in vivo.en_US
dc.language.isoEnglishen_US
dc.source.titleJournal of Biological Chemistryen_US
dc.relation.isversionofhttp://dx.doi.org/10.1074/jbc.M112.378687en_US
dc.subjectBacterial artificial chromosomesen_US
dc.subjectCD8 T lymphocytesen_US
dc.subjectDexamethasonesen_US
dc.subjectGene transcriptionsen_US
dc.subjectGlucocorticoidsen_US
dc.subjectGrowth factoren_US
dc.subjectIn-vivoen_US
dc.subjectInterleukin-7en_US
dc.subjectMicroarray expressionsen_US
dc.subjectReceptor expressionen_US
dc.subjectRetroviral transductionen_US
dc.subjectT cellsen_US
dc.subjectThymocytesen_US
dc.subjectTranscriptional repressorsen_US
dc.subjectTransgeneen_US
dc.subjectTransgenic miceen_US
dc.subjectUp-regulationen_US
dc.titleCD8 lineage-specific regulation of interleukin-7 receptor expression by the transcriptional repressor Gfi1en_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.citation.spage34386en_US
dc.citation.epage34399en_US
dc.citation.volumeNumber287en_US
dc.citation.issueNumber41en_US
dc.identifier.doi10.1074/jbc.M112.378687en_US
dc.publisherThe American Society for Biochemistry and Molecular Biology, Inc.en_US


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