Transmission Near-Field Scanning Optical Microscopy Investigation on Cellular Uptake Behavior of Iron Oxide Nanoparticles
Author
Zhang, Y.
Kyle J.R.
Penchev, M.
Yazdanpanah V.
Yu J.
Li, Y.
Yang, M.
Budak G.
Özbay, Ekmel
Ozkan, M.
Ozkan, C.S.
Date
2012Source Title
BioNanoScience
Print ISSN
21911630
Volume
2
Issue
3
Pages
135 - 143
Language
English
Type
ArticleItem Usage Stats
149
views
views
125
downloads
downloads
Abstract
Cellular uptake behavior of iron oxide nanoparticles is investigated using a transmission near-field scanning optical microscopy (NSOM) without the need of fluorescent labeling. By using the transmission NSOM system, we could simultaneously explore the near-field optical analysis of the cell interior and record the topographic information of the cell surface. The cell endocytosis of iron oxide nanoparticles by normal breast MCF10A cells is first studied by this transmission NSOM system, and this dual functional nanoscale-resolution microscopy shows the capability of mapping the spatial localization of nanoparticles in/outside cell surface without the need of fluorescence labeling. Nanoscale optical signature patterns for iron oxide nanoparticle-loaded vesicles inside the cells were observed and analyzed. © Springer Science+Business Media, LLC 2012.
Keywords
AFMEndocytosis
Iron oxide
MCF10A
Nanoparticle
NSOM
AFM
Endocytosis
Fluorescence labeling
Fluorescent labeling
Iron oxide nanoparticle
MCF10A
Spatial localization
Topographic information
Cell membranes
Cytology
Fluorescence
Iron oxides
Metal nanoparticles
Molecular biology
Nanoparticles
Nanotechnology
Near field scanning optical microscopy
fluorescent dye
iron oxide
nanoparticle
article
controlled study
detoxification
drug delivery system
drug uptake
endocytosis
fluorescence
human
human cell
kinetics
light scattering
nuclear magnetic resonance imaging
polarimetry
scanning electron microscopy
scanning near field optical microscopy
tissue repair
topography
zeta potential
Permalink
http://hdl.handle.net/11693/21210Published Version (Please cite this version)
http://dx.doi.org/10.1007/s12668-012-0043-8Collections
Related items
Showing items related by title, author, creator and subject.
-
Non-radiative resonance energy transfer in bi-polymer nanoparticles of fluorescent conjugated polymers
Ozel I.O.; Ozel, T.; Demir, Hilmi Volkan; Tuncel, D. (Optical Society of American (OSA), 2010)This work demonstrates the comparative studies of non-radiative resonance energy transfer in bi-polymer nanoparticles based on fluorescent conjugated polymers. For this purpose, poly[(9,9-dihexylfluorene) (PF) as a donor ... -
Ultrasensitive electrospun fluorescent nanofibrous membrane for rapid visual colorimetric detection of H2O2
Senthamizhan A.; Balusamy, B.; Aytac Z.; Uyar, Tamer (Springer Verlag, 2016-02)We report herein a flexible fluorescent nanofibrous membrane (FNFM) prepared by decorating the gold nanocluster (AuNC) on electrospun polysulfone nanofibrous membrane for rapid visual colorimetric detection of H2O2. The ... -
White-emitting conjugated polymer nanoparticles with cross-linked shell for mechanical stability and controllable photometric properties in color-conversion LED applications
Park, Eun-Ju; Erdem, T.; Ibrahimova, V.; Nizamoglu, S.; Demir, Hilmi Volkan; Tuncel, D. (2011)We report on the synthesis and characterization of water-dispersible, mechanically stable conjugated polymer nanoparticles (CPNs) in shelled architecture with tunable emission and controllable photometric properties via ...