Chitosan scaffolds with BMP-6 loaded alginate microspheres for periodontal tissue engineering
Author
Soran, Z.
Aydin, R.S.T.
Gumusderelioglu, M.
Date
2012Source Title
Journal of Microencapsulation
Print ISSN
0265-2048
Volume
29
Issue
8
Pages
770 - 780
Language
English
Type
ArticleItem Usage Stats
127
views
views
139
downloads
downloads
Abstract
The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow size distribution were produced by optimising electrospraying conditions. The addition of these microspheres to chitosan gels produced a novel scaffold in which not only the pore sizes and interconnectivity were preserved, but also a controlled release vehicle was generated. Loading capacity was adjusted as 50ng or 100ng BMP-6 for each scaffold and the controlled release behaviour of BMP-6 from chitosan scaffolds was observed during seven days. Cell culture studies were carried out with rat mesenchymal stem cells derived from bone marrow in three groups; chitosan scaffolds, chitosan scaffolds containing BMP-6-loaded alginate microspheres and chitosan scaffolds with free BMP-6 in culture medium. Results showed that controlled delivery of BMP-6 from alginate microspheres has a significant effect on osteogenic differentiation. © 2012 Informa UK Ltd All rights reserved.
Keywords
Alginate microspheresBMP-6
Chitosan scaffold
Mesenchymal stem cells
Periodontal tissue engineering
alginic acid
bone morphogenetic protein 6
chitosan
microsphere
animal cell
article
bone marrow
cell culture
cell differentiation
controlled release formulation
culture medium
drug release
electrospray
mesenchymal stem cell
nonhuman
periodontium
rat
tissue engineering
Alginates
Animals
Bone Morphogenetic Protein 6
Chitosan
Culture Media
Glucuronic Acid
Hexuronic Acids
Mesenchymal Stromal Cells
Microscopy, Electron, Scanning
Microspheres
Periodontium
Rats
Tissue Engineering