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      Inflammasome induction and immunostimulatory effects of CpG-ODN loaded liposomes containing DC-cholesterol

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      Author
      Bayyurt, B.
      Gürsel I.
      Date
      2013
      Source Title
      Turkish Journal of Immunology
      Print ISSN
      1301109X
      Publisher
      Turkish Society of Immunology
      Volume
      2
      Issue
      2
      Pages
      21 - 28
      Language
      Turkish
      Type
      Article
      Item Usage Stats
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      148
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      Abstract
      Objectives: This study aims to investigate the effects of cholesterol content and cationic property of liposomes on immune response. Materials and methods: Liposomes containing high amounts of 3β-[N-(N',N'-dimethylaminoethane)-carbamoyl] cholesterol hydrochloride (DC-cholesterol) were prepared and loaded with K- and D-type CpG oligonucleotide (CpG-ODN) via dehydration-rehydration (DRV) method. After splenocytes and peritoneal exudate cells (PECs) primed with lipopolysaccharide (LPS) was incubated either with free or liposomal CpG-ODN counterparts, supernatants were collected and used in cytokine (IFN-g, IL-1γ and IL-1β) ELISA. Additionally, supernatants of PECs primed with LPS and stimulated with liposomes containing different doses of DC-cholesterol were collected and used in IL-1β ELISA assay. Results: Low-dose CpG-ODN loaded liposomal formulations induced higher immune activation than free CpG-ODN at the same dose. While high-dose liposomal CpG-ODN formulations decreased pro-inflammatory cytokine production in splenocytes, they increased the secretion of IL-1β. Inflammasome activation was increased in a dose dependent manner when PECs primed with LPS were incubated with only liposomes. Varying lipid molar ratios of DC-Cholesterol containing liposomes increased IL-1β production based on increasing lipid molar ratio. Conclusion: This study revealed that type and lipid ratio of liposomes may alter the cellular efficacy of the loaded immune-stimulatory agent and may initiate inflammasome activation. © 2014 Turkish Journal of Immunology.
      Keywords
      CpG-ODN
      DC-cholesterol
      Immune response
      Inflammasome
      Liposome
      3 beta [n [ n',n' dimethylaminoethane] caramoyl]cholesterol hydrochloride
      cationic liposome
      CpG oligodeoxynucleotide
      gamma interferon
      inflammasome
      interleukin 12
      interleukin 1beta
      lipopolysaccharide
      liposome
      unclassified drug
      Article
      cholesterol synthesis
      cytokine release
      dehydration
      enzyme linked immunosorbent assay
      human
      immune response
      immunostimulation
      peritoneal exudate cell
      rehydration
      spleen cell
      supernatant
      Permalink
      http://hdl.handle.net/11693/21160
      Published Version (Please cite this version)
      http://dx.doi.org/10.5606/tji.2014.324
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