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      Production and structural characterization of biosurfactant produced by newly isolated staphylococcus xylosus STF1 from petroleum contaminated soil

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      Author
      Keskin, N. O. S.
      Han, D.
      Ozkan A.D.
      Angun, P.
      Umu, O. C. O.
      Tekinay, T.
      Date
      2015
      Source Title
      Journal of Petroleum Science and Engineering
      Print ISSN
      0920-4105
      Publisher
      Elsevier BV
      Volume
      133
      Pages
      689 - 694
      Language
      English
      Type
      Article
      Item Usage Stats
      186
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      228
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      Abstract
      Petroleum-contaminated soil was used to isolate and characterize biosurfactant producing bacteria. The strain could produce higher amount of biosurfactant in medium supplemented with motor oil as sole source of carbon and energy. A new biosurfactant producing bacterium, designated as Staphylococcus xylosus STF1 based on morphological, physiological, biochemical tests and 16S rRNA gene sequencing. The isolated bacterium was first screened for the ability to produce biosurfactant. Partial sequence of STF1 strain of 16S rDNA gene was highly similar to those of various members of the family Staphylococcaceae. Biochemical characterizations including FT-IR, Raman spectroscopy and Mass spectroscopy studies suggested the biosurfactant to be lipopeptide. Study also confirmed that the cell free supernatant exhibited high emulsifying activity against the different hydrocarbons. Moreover, the partially purified biosurfactant exhibited antimicrobial activity by inhibiting the growth of several bacterial species. The strain could be a potential candidate for the production of polypeptide biosurfactant which could be useful in a variety of biotechnological and industrial processes, particularly in the food and oil industry. © 2015 Elsevier B.V.
      Keywords
      Biosurfactant
      FT-IR
      Lipopeptide
      Raman spectroscopy
      Staphylococcus
      Bacteria
      Carbon
      Emulsification
      Genes
      Mass spectrometry
      Oils and fats
      Raman spectroscopy
      RNA
      Soil pollution
      Surface active agents
      Anti-microbial activity
      Bio surfactant
      Biochemical characterization
      Lipopeptides
      Petroleum-contaminated soil
      Staphylococcus
      Structural characterization
      Biomolecules
      Bacterium
      DNA
      FTIR spectroscopy
      Mass spectrometry
      Microbial activity
      Peptide
      Petroleum
      Surfactant
      Bacteria (microorganisms)
      Staphylococcaceae
      Staphylococcus
      Staphylococcus xylosus
      Permalink
      http://hdl.handle.net/11693/21103
      Published Version (Please cite this version)
      https://doi.org/10.1016/j.petrol.2015.07.011
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      • Institute of Materials Science and Nanotechnology (UNAM) 1775
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