Production and structural characterization of biosurfactant produced by newly isolated staphylococcus xylosus STF1 from petroleum contaminated soil
Author
Keskin, N. O. S.
Han, D.
Ozkan A.D.
Angun, P.
Umu, O. C. O.
Tekinay, T.
Date
2015Source Title
Journal of Petroleum Science and Engineering
Print ISSN
0920-4105
Publisher
Elsevier BV
Volume
133
Pages
689 - 694
Language
English
Type
ArticleItem Usage Stats
186
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views
228
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downloads
Abstract
Petroleum-contaminated soil was used to isolate and characterize biosurfactant producing bacteria. The strain could produce higher amount of biosurfactant in medium supplemented with motor oil as sole source of carbon and energy. A new biosurfactant producing bacterium, designated as Staphylococcus xylosus STF1 based on morphological, physiological, biochemical tests and 16S rRNA gene sequencing. The isolated bacterium was first screened for the ability to produce biosurfactant. Partial sequence of STF1 strain of 16S rDNA gene was highly similar to those of various members of the family Staphylococcaceae. Biochemical characterizations including FT-IR, Raman spectroscopy and Mass spectroscopy studies suggested the biosurfactant to be lipopeptide. Study also confirmed that the cell free supernatant exhibited high emulsifying activity against the different hydrocarbons. Moreover, the partially purified biosurfactant exhibited antimicrobial activity by inhibiting the growth of several bacterial species. The strain could be a potential candidate for the production of polypeptide biosurfactant which could be useful in a variety of biotechnological and industrial processes, particularly in the food and oil industry. © 2015 Elsevier B.V.
Keywords
BiosurfactantFT-IR
Lipopeptide
Raman spectroscopy
Staphylococcus
Bacteria
Carbon
Emulsification
Genes
Mass spectrometry
Oils and fats
Raman spectroscopy
RNA
Soil pollution
Surface active agents
Anti-microbial activity
Bio surfactant
Biochemical characterization
Lipopeptides
Petroleum-contaminated soil
Staphylococcus
Structural characterization
Biomolecules
Bacterium
DNA
FTIR spectroscopy
Mass spectrometry
Microbial activity
Peptide
Petroleum
Surfactant
Bacteria (microorganisms)
Staphylococcaceae
Staphylococcus
Staphylococcus xylosus
Permalink
http://hdl.handle.net/11693/21103Published Version (Please cite this version)
https://doi.org/10.1016/j.petrol.2015.07.011Collections
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