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      • Department of Molecular Biology and Genetics
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      Effect of double growth factor release on cartilage tissue engineering

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      Author(s)
      Ertan, A.B.
      Yilgor P.
      Bayyurt, B.
      Çalikoǧlu, A.C.
      Kaspar Ç.
      Kök F.N.
      Kose G.T.
      Hasirci V.
      Date
      2013
      Source Title
      Journal of Tissue Engineering and Regenerative Medicine
      Print ISSN
      19326254
      Volume
      7
      Issue
      2
      Pages
      149 - 160
      Language
      English
      Type
      Article
      Item Usage Stats
      236
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      305
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      Abstract
      The effects of double release of insulin-like growth factor I (IGF-I) and growth factor β1 (TGF-β1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF-β1, respectively. On tissue culture polystyrene (TCPS), TGF-β1 released from PNIPAM nanoparticles was found to have a significant effect on proliferation, while IGF-I encouraged differentiation, as shown by collagen type II deposition. The study was then conducted on macroporous (pore size 200-400μm) PLGA scaffolds. It was observed that the combination of IGF-I and TGF-β1 yielded better results in terms of collagen type II and aggrecan expression than GF-free and single GF-containing applications. It thus appears that gradual release of a combination of growth factors from nanoparticles could make a significant contribution to the quality of the engineered cartilage tissue. © 2011 John Wiley & Sons, Ltd.
      Keywords
      Cartilage tissue engineering
      Cell differentiation
      Growth factors
      Mesenchymal stem cells
      Peptide and protein delivery
      aggrecan
      collagen type 2
      nanoparticle
      poly(n isopropylacrylamide)
      polyglactin
      polystyrene
      somatomedin C
      transforming growth factor beta1
      animal cell
      animal experiment
      animal tissue
      article
      bone marrow cell
      cartilage
      cell differentiation
      cell growth
      cell proliferation
      controlled study
      male
      mesenchymal stem cell
      nonhuman
      particle size
      priority journal
      rat
      tissue culture
      tissue engineering
      Acrylamides
      Aggrecans
      Animals
      Cartilage
      Cattle
      Cell Proliferation
      Collagen
      Collagen Type II
      Extracellular Matrix
      Glycosaminoglycans
      Insulin-Like Growth Factor I
      Lactic Acid
      Male
      Microscopy, Confocal
      Nanoparticles
      Particle Size
      Polyglycolic Acid
      Polymers
      Rats
      Rats, Sprague-Dawley
      Real-Time Polymerase Chain Reaction
      Serum Albumin, Bovine
      Tissue Engineering
      Tissue Scaffolds
      Transforming Growth Factor beta1
      Permalink
      http://hdl.handle.net/11693/21100
      Published Version (Please cite this version)
      http://dx.doi.org/10.1002/term.509
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      • Department of Molecular Biology and Genetics 512
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