Effect of double growth factor release on cartilage tissue engineering
Date
2013Source Title
Journal of Tissue Engineering and Regenerative Medicine
Print ISSN
19326254
Volume
7
Issue
2
Pages
149 - 160
Language
English
Type
ArticleItem Usage Stats
236
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305
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Abstract
The effects of double release of insulin-like growth factor I (IGF-I) and growth factor β1 (TGF-β1) from nanoparticles on the growth of bone marrow mesenchymal stem cells and their differentiation into cartilage cells were studied on PLGA scaffolds. The release was achieved by using nanoparticles of poly(lactic acid-co-glycolic acid) (PLGA) and poly(N-isopropylacrylamide) (PNIPAM) carrying IGF-I and TGF-β1, respectively. On tissue culture polystyrene (TCPS), TGF-β1 released from PNIPAM nanoparticles was found to have a significant effect on proliferation, while IGF-I encouraged differentiation, as shown by collagen type II deposition. The study was then conducted on macroporous (pore size 200-400μm) PLGA scaffolds. It was observed that the combination of IGF-I and TGF-β1 yielded better results in terms of collagen type II and aggrecan expression than GF-free and single GF-containing applications. It thus appears that gradual release of a combination of growth factors from nanoparticles could make a significant contribution to the quality of the engineered cartilage tissue. © 2011 John Wiley & Sons, Ltd.
Keywords
Cartilage tissue engineeringCell differentiation
Growth factors
Mesenchymal stem cells
Peptide and protein delivery
aggrecan
collagen type 2
nanoparticle
poly(n isopropylacrylamide)
polyglactin
polystyrene
somatomedin C
transforming growth factor beta1
animal cell
animal experiment
animal tissue
article
bone marrow cell
cartilage
cell differentiation
cell growth
cell proliferation
controlled study
male
mesenchymal stem cell
nonhuman
particle size
priority journal
rat
tissue culture
tissue engineering
Acrylamides
Aggrecans
Animals
Cartilage
Cattle
Cell Proliferation
Collagen
Collagen Type II
Extracellular Matrix
Glycosaminoglycans
Insulin-Like Growth Factor I
Lactic Acid
Male
Microscopy, Confocal
Nanoparticles
Particle Size
Polyglycolic Acid
Polymers
Rats
Rats, Sprague-Dawley
Real-Time Polymerase Chain Reaction
Serum Albumin, Bovine
Tissue Engineering
Tissue Scaffolds
Transforming Growth Factor beta1
Permalink
http://hdl.handle.net/11693/21100Published Version (Please cite this version)
http://dx.doi.org/10.1002/term.509Collections
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