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dc.contributor.authorBayramoglu, G.en_US
dc.contributor.authorBitirim, V.en_US
dc.contributor.authorTunali, Y.en_US
dc.contributor.authorArica, M. Y.en_US
dc.contributor.authorAkcali, K. C.en_US
dc.date.accessioned2016-02-08T09:40:21Z
dc.date.available2016-02-08T09:40:21Z
dc.date.issued2013en_US
dc.identifier.issn0928-4931
dc.identifier.urihttp://hdl.handle.net/11693/21055
dc.description.abstractCopolymerization of 2-hydroxyethylmethacrylate (HEMA) with glycidylmethacrylate (GMA) in the presence of α-α′- azoisobisbutyronitrile (AIBN) resulted in the formation of hydrogel films carrying reactive epoxy groups. Thirteen kinds of different molecules with pendant NH2 group were used for modifications of the p(HEMA-GMA) films. The NH2 group served as anchor binding site for immobilization of functional groups on the hydrogel film via direct epoxy ring opening reaction. The modified hydrogel films were characterized by FTIR, and contact angle studies. In addition, mechanical properties of the hydrogel films were studied, and modified hydrogel films showed improved mechanical properties compared with the non-modified film, but they are less elastic than the non-modified film. The biological activities of these films such as platelet adhesion, red blood cells hemolysis, and swelling behavior were studied. The effect of modified hydrogel films, including NH2, (using different aliphatic CH2 chain lengths) CH3, SO3H, aromatic groups with substituted OH and COOH groups, and amino acids were also investigated on the adhesion, morphology and survival of rat mesenchymal stem cells (MSCs). The MTT colorimetric assay reveals that the p(HEMA-GMA)-GA-AB, p(HEMA-GMA)-GA-Phe, p(HEMA-GMA)-GA-Trp, p(HEMA-GMA)-GA-Glu formulations have an excellent biocompatibility to promote the cell adhesion and growth. We anticipate that the fabricated p(HEMA-GMA) based hydrogel films with controllable surface chemistry and good stable swelling ratio may find extensive applications in future development of tissue engineering scaffold materials, and in various biotechnological areas. © 2012 Elsevier B.V.en_US
dc.language.isoEnglishen_US
dc.source.titleMaterials Science and Engineering Cen_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.msec.2012.11.004en_US
dc.subjectBiocompatible polymersen_US
dc.subjectBlood cell adhesionen_US
dc.subjectContact angleen_US
dc.subjectHemolysisen_US
dc.subjectHydrogelsen_US
dc.subjectRat mesenchymal stem cellsen_US
dc.titlePoly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cellsen_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.citation.spage801en_US
dc.citation.epage810en_US
dc.citation.volumeNumber33en_US
dc.citation.issueNumber2en_US
dc.identifier.doi10.1016/j.msec.2012.11.004en_US
dc.publisherElsevieren_US


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