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      Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

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      Author(s)
      Bayramoglu, G.
      Bitirim, V.
      Tunali, Y.
      Arica, M. Y.
      Akcali, K. C.
      Date
      2013
      Source Title
      Materials Science and Engineering C
      Print ISSN
      0928-4931
      Publisher
      Elsevier
      Volume
      33
      Issue
      2
      Pages
      801 - 810
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      Copolymerization of 2-hydroxyethylmethacrylate (HEMA) with glycidylmethacrylate (GMA) in the presence of α-α′- azoisobisbutyronitrile (AIBN) resulted in the formation of hydrogel films carrying reactive epoxy groups. Thirteen kinds of different molecules with pendant NH2 group were used for modifications of the p(HEMA-GMA) films. The NH2 group served as anchor binding site for immobilization of functional groups on the hydrogel film via direct epoxy ring opening reaction. The modified hydrogel films were characterized by FTIR, and contact angle studies. In addition, mechanical properties of the hydrogel films were studied, and modified hydrogel films showed improved mechanical properties compared with the non-modified film, but they are less elastic than the non-modified film. The biological activities of these films such as platelet adhesion, red blood cells hemolysis, and swelling behavior were studied. The effect of modified hydrogel films, including NH2, (using different aliphatic CH2 chain lengths) CH3, SO3H, aromatic groups with substituted OH and COOH groups, and amino acids were also investigated on the adhesion, morphology and survival of rat mesenchymal stem cells (MSCs). The MTT colorimetric assay reveals that the p(HEMA-GMA)-GA-AB, p(HEMA-GMA)-GA-Phe, p(HEMA-GMA)-GA-Trp, p(HEMA-GMA)-GA-Glu formulations have an excellent biocompatibility to promote the cell adhesion and growth. We anticipate that the fabricated p(HEMA-GMA) based hydrogel films with controllable surface chemistry and good stable swelling ratio may find extensive applications in future development of tissue engineering scaffold materials, and in various biotechnological areas. © 2012 Elsevier B.V.
      Keywords
      Biocompatible polymers
      Blood cell adhesion
      Contact angle
      Hemolysis
      Hydrogels
      Rat mesenchymal stem cells
      Permalink
      http://hdl.handle.net/11693/21055
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.msec.2012.11.004
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