Induction of potent protection against acute and latent herpes simplex virus infection in mice vaccinated with dendritic cells
Author
Ghasemi, M.
Erturk, M.
Buruk, K.
Sonmez, M.
Date
2013Source Title
Cytotherapy
Print ISSN
14653249
Volume
15
Issue
3
Pages
352 - 361
Language
English
Type
ArticleItem Usage Stats
113
views
views
114
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downloads
Abstract
Background aims. Dendritic cells (DCs) are the most potent antigen presenting cells of the immune system and have been under intense study with regard to their use in immunotherapy against cancer and infectious disease agents. In the present study, DCs were employed to assess their value in protection against live virus challenge in an experimental model using lethal and latent herpes simplex virus (HSV) infection in Balb/c mice. Methods. DCs obtained ex vivo in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4 were loaded with HSV-1 proteins (DC/HSV-1 vaccine). Groups of mice were vaccinated twice, 7 days apart, via subcutaneous, intraperitoneal or intramuscular routes with DC/HSV-1 and with mock (DC without virus protein) and positive (alum adjuvanted HSV-1 proteins [HSV-1/ALH]) control vaccines. After measuring anti-HSV-1 antibody levels in blood samples, mice were given live HSV-1 intraperitoneally or via ear pinna to assess the protection level of the vaccines with respect to lethal or latent infection challenge. Results. Intramuscular, but not subcutaneous or intraperitoneal, administration of DC/HSV-1 vaccine provided complete protection against lethal challenge and establishment of latent infection as assessed by death and virus recovery from the trigeminal ganglia. It was also shown that the immunity was not associated with antibody production because DC/HSV-1 vaccine, as opposed to HSV-1/ALH vaccine, produced very little, if any, HSV-1-specific antibody. Conclusions. Overall, our results may have some impact on the design of vaccines against genital HSV as well as chronic viral infections such as hepatitis B virus, hepatitis C virus and human immunodeficiency virus. © 2013, International Society for Cellular Therapy.
Keywords
Dendritic cellsHerpes simplex virus
Mice
Vaccine
dendritic cell vaccine
granulocyte macrophage colony stimulating factor
herpes simplex vaccine
interleukin 4
animal cell
animal experiment
animal model
antibody production
article
controlled study
ex vivo study
herpes simplex
immunization
mouse
nonhuman
priority journal
treatment response
trigeminus ganglion
virus immunity
Animals
Antibodies, Viral
Dendritic Cells
Granulocyte-Macrophage Colony-Stimulating Factor
Herpes Simplex
Herpes Simplex Virus Vaccines
Humans
Immunotherapy
Interleukin-4
Mice
Simplexvirus
Viral Envelope Proteins
Permalink
http://hdl.handle.net/11693/21049Published Version (Please cite this version)
http://dx.doi.org/10.1016/j.jcyt.2012.11.012Collections
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