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      Patterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cells

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      Author
      Bitirim, Verda Ceylan
      Küçükayan-Doğu, Gökçe
      Bengü, Erman
      Akçalı, Kamil Can
      Date
      2013
      Source Title
      Materials Science and Engineering C
      Print ISSN
      0928-4931
      Publisher
      Elsevier
      Volume
      33
      Issue
      5
      Pages
      3054 - 3060
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      We investigated the cellular adhesive features of mesenchymal stem cells (MSC) on non-coated and collagen coated patterned and vertically aligned carbon nanotube (CNT) structures mimicking the natural extra cellular matrix (ECM). Patterning was achieved using the elasto-capillary induced by water treatment on the CNT arrays. After confirmation with specific markers both at transcript and protein levels, MSCs from different passages were seeded on either collagen coated or non-coated patterned CNTs. Adhesion and growth of MSCs on the patterned CNT arrays were examined using scanning electron microscopy image analysis and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. The highest MSC count was observed on the non-coated patterned CNTs at passage zero, while decreasing numbers of MSCs were found at the later passages. Similarly, MTT assay results also revealed a decrease in the viability of the MSCs for the later passages. Overall, the cell count and viability experiments indicated that MSCs were able to better attach to non-coated patterned CNTs compared to those coated with collagen. Therefore, the patterned CNT surfaces can be potentially used as a scaffold mimicking the ECM environment for MSC growth which presents an alternative approach to MSC-based transplantation therapy applications.
      Keywords
      Vertically aligned carbon nanotube
      Stem cell
      Extra cellular matrix
      Viability
      Patterning
      Collagen
      Permalink
      http://hdl.handle.net/11693/20916
      Published Version (Please cite this version)
      http://dx.doi.org/10.1016/j.msec.2013.03.044
      Collections
      • Department of Chemistry 594
      • Department of Molecular Biology and Genetics 426
      • Genetics and Biotechnology Research Center (BİLGEN) 16
      • Institute of Materials Science and Nanotechnology (UNAM) 1775
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