Patterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cells
Author
Bitirim, Verda Ceylan
Küçükayan-Doğu, Gökçe
Bengü, Erman
Akçalı, Kamil Can
Date
2013Source Title
Materials Science and Engineering C
Print ISSN
0928-4931
Publisher
Elsevier
Volume
33
Issue
5
Pages
3054 - 3060
Language
English
Type
ArticleItem Usage Stats
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Abstract
We investigated the cellular adhesive features of mesenchymal stem cells (MSC) on non-coated and collagen coated patterned and vertically aligned carbon nanotube (CNT) structures mimicking the natural extra cellular matrix (ECM). Patterning was achieved using the elasto-capillary induced by water treatment on the CNT arrays. After confirmation with specific markers both at transcript and protein levels, MSCs from different passages were seeded on either collagen coated or non-coated patterned CNTs. Adhesion and growth of MSCs on the patterned CNT arrays were examined using scanning electron microscopy image analysis and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. The highest MSC count was observed on the non-coated patterned CNTs at passage zero, while decreasing numbers of MSCs were found at the later passages. Similarly, MTT assay results also revealed a decrease in the viability of the MSCs for the later passages. Overall, the cell count and viability experiments indicated that MSCs were able to better attach to non-coated patterned CNTs compared to those coated with collagen. Therefore, the patterned CNT surfaces can be potentially used as a scaffold mimicking the ECM environment for MSC growth which presents an alternative approach to MSC-based transplantation therapy applications.
Keywords
Vertically aligned carbon nanotubeStem cell
Extra cellular matrix
Viability
Patterning
Collagen