Patterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cells
Please cite this item using this persistent URLhttp://hdl.handle.net/11693/20916
Materials Science and Engineering C
- Research Paper 
We investigated the cellular adhesive features of mesenchymal stem cells (MSC) on non-coated and collagen coated patterned and vertically aligned carbon nanotube (CNT) structures mimicking the natural extra cellular matrix (ECM). Patterning was achieved using the elasto-capillary induced by water treatment on the CNT arrays. After confirmation with specific markers both at transcript and protein levels, MSCs from different passages were seeded on either collagen coated or non-coated patterned CNTs. Adhesion and growth of MSCs on the patterned CNT arrays were examined using scanning electron microscopy image analysis and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. The highest MSC count was observed on the non-coated patterned CNTs at passage zero, while decreasing numbers of MSCs were found at the later passages. Similarly, MTT assay results also revealed a decrease in the viability of the MSCs for the later passages. Overall, the cell count and viability experiments indicated that MSCs were able to better attach to non-coated patterned CNTs compared to those coated with collagen. Therefore, the patterned CNT surfaces can be potentially used as a scaffold mimicking the ECM environment for MSC growth which presents an alternative approach to MSC-based transplantation therapy applications. © 2013 Elsevier B.V.
Showing items related by title, author, creator and subject.
Rana, K.; Kucukayan-Dogu G.; Sen H.S.; Boothroyd, C.; Gulseren O.; Bengu, E. (2012)Vertically aligned carbon nanotube (VA-CNT) arrays have been synthesized with lithium (Li) intercalation through an alcohol-catalyzed chemical vapor deposition technique by using a Li-containing catalyst. Scanning electron ...
Soran, Z.; Aydin, R.S.T.; Gumusderelioglu, M. (2012)The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow ...
Bioactive surface design based on functional composite electrospun nanofibers for biomolecule immobilization and biosensor applications Demirci Uzun, S.; Kayaci F.; Uyar, T.; Timur, S.; Toppare L. (American Chemical Society, 2014)The combination of nanomaterials and conducting polymers attracted remarkable attention for development of new immobilization matrices for enzymes. Hereby, an efficient surface design was investigated by modifying the ...