β-Tubulin mRNA expression profiles during long-term memory formation in classically conditioned C. elegans
Author
Erdoğan, Mutlu
Şahin, E.
Date
2013Source Title
Journal of Neurological Sciences
Print ISSN
1300-1817
Publisher
Ege Üniversitesi
Volume
30
Issue
2
Pages
296 - 304
Language
English; Turkish
Type
ArticleItem Usage Stats
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Abstract
We report on the mRNA expression profiles of β-tubulin genes in Caenorhabditis elegans during the process of classical conditioning, a type of associative learning. In this research, based on the model that suggests the formation of new synaptic connections for long-term memory storage by means of cytoskeletal rearrangement, we measure the expressional changes on the β-tubulin mRNA levels via quantitative real-time PCR. Using this method, we find that β-tubulin mRNA levels do not significantly change in C. elegans during classical conditioning and long-term memory formation process.
Keywords
C. elegansClassical conditioning
Learning
Long-term memory
Memory
Tubulin
Beta tubulin
Animal experiment
Caenorhabditis elegans
Chemotaxis
Conditioning
Controlled study
Long term memory
Nerve cell plasticity
Nonhuman
Protein expression
Real time polymerase chain reaction
RNA isolation
Sensory nerve cell
Spectrophotometry