Coiled-Coil Domain Containing Protein 124 Is a Novel Centrosome and Midbody Protein That Interacts with the Ras-Guanine Nucleotide Exchange Factor 1B and Is Involved in Cytokinesis
Author
Telkoparan P.
Erkek, S.
Yaman, E.
Alotaibi H.
Bayik, D.
Tazebay, U.H.
Date
2013Source Title
PLoS ONE
Print ISSN
19326203
Volume
8
Issue
7
Language
English
Type
ArticleItem Usage Stats
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Show full item recordAbstract
Cytokinetic abscission is the cellular process leading to physical separation of two postmitotic sister cells by severing the intercellular bridge. The most noticeable structural component of the intercellular bridge is a transient organelle termed as midbody, localized at a central region marking the site of abscission. Despite its major role in completion of cytokinesis, our understanding of spatiotemporal regulation of midbody assembly is limited. Here, we report the first characterization of coiled-coil domain-containing protein-124 (Ccdc124), a eukaryotic protein conserved from fungi-to-man, which we identified as a novel centrosomal and midbody protein. Knockdown of Ccdc124 in human HeLa cells leads to accumulation of enlarged and multinucleated cells; however, centrosome maturation was not affected. We found that Ccdc124 interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B), establishing a functional link between cytokinesis and activation of localized Rap2 signaling at the midbody. Our data indicate that Ccdc124 is a novel factor operating both for proper progression of late cytokinetic stages in eukaryotes, and for establishment of Rap2 signaling dependent cellular functions proximal to the abscission site. © 2013 Telkoparan et al.
Keywords
coiled coil domain containing protein 124guanine nucleotide binding protein
guanine nucleotide exchange factor
guanine nucleotide exchange factor 1B
membrane protein
Rap2 protein
unclassified drug
article
cell function
centrosome
controlled study
cytokinesis
eukaryote
gene silencing
HeLa cell
human
human cell
multinuclear cell
nucleotide sequence
protein interaction
signal transduction
Carrier Proteins
Centrosome
Cytokinesis
Gene Expression
Gene Knockdown Techniques
HeLa Cells
Humans
Intracellular Signaling Peptides and Proteins
Organ Specificity
Organelles
Protein Binding
Protein Interaction Mapping
Protein Transport
rap GTP-Binding Proteins
ras Guanine Nucleotide Exchange Factors
RNA Interference
RNA, Messenger
Telophase
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http://hdl.handle.net/11693/20893Published Version (Please cite this version)
http://dx.doi.org/10.1371/journal.pone.0069289Collections
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