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      Coiled-Coil Domain Containing Protein 124 Is a Novel Centrosome and Midbody Protein That Interacts with the Ras-Guanine Nucleotide Exchange Factor 1B and Is Involved in Cytokinesis

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      Author
      Telkoparan P.
      Erkek, S.
      Yaman, E.
      Alotaibi H.
      Bayik, D.
      Tazebay, U.H.
      Date
      2013
      Source Title
      PLoS ONE
      Print ISSN
      19326203
      Volume
      8
      Issue
      7
      Language
      English
      Type
      Article
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      Abstract
      Cytokinetic abscission is the cellular process leading to physical separation of two postmitotic sister cells by severing the intercellular bridge. The most noticeable structural component of the intercellular bridge is a transient organelle termed as midbody, localized at a central region marking the site of abscission. Despite its major role in completion of cytokinesis, our understanding of spatiotemporal regulation of midbody assembly is limited. Here, we report the first characterization of coiled-coil domain-containing protein-124 (Ccdc124), a eukaryotic protein conserved from fungi-to-man, which we identified as a novel centrosomal and midbody protein. Knockdown of Ccdc124 in human HeLa cells leads to accumulation of enlarged and multinucleated cells; however, centrosome maturation was not affected. We found that Ccdc124 interacts with the Ras-guanine nucleotide exchange factor 1B (RasGEF1B), establishing a functional link between cytokinesis and activation of localized Rap2 signaling at the midbody. Our data indicate that Ccdc124 is a novel factor operating both for proper progression of late cytokinetic stages in eukaryotes, and for establishment of Rap2 signaling dependent cellular functions proximal to the abscission site. © 2013 Telkoparan et al.
      Keywords
      coiled coil domain containing protein 124
      guanine nucleotide binding protein
      guanine nucleotide exchange factor
      guanine nucleotide exchange factor 1B
      membrane protein
      Rap2 protein
      unclassified drug
      article
      cell function
      centrosome
      controlled study
      cytokinesis
      eukaryote
      gene silencing
      HeLa cell
      human
      human cell
      multinuclear cell
      nucleotide sequence
      protein interaction
      signal transduction
      Carrier Proteins
      Centrosome
      Cytokinesis
      Gene Expression
      Gene Knockdown Techniques
      HeLa Cells
      Humans
      Intracellular Signaling Peptides and Proteins
      Organ Specificity
      Organelles
      Protein Binding
      Protein Interaction Mapping
      Protein Transport
      rap GTP-Binding Proteins
      ras Guanine Nucleotide Exchange Factors
      RNA Interference
      RNA, Messenger
      Telophase
      Permalink
      http://hdl.handle.net/11693/20893
      Published Version (Please cite this version)
      http://dx.doi.org/10.1371/journal.pone.0069289
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      • Department of Molecular Biology and Genetics 409

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