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      Na+/I-symporter and type 3 iodothyronine deiodinase gene expression in amniotic membrane and placenta and its relationship to maternal thyroid hormones

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      Author
      Akturk, M.
      Oruc, A.S.
      Danisman, N.
      Erkek, S.
      Buyukkagnici, U.
      Unlu, E.
      Tazebay, U.H.
      Date
      2013
      Source Title
      Biological Trace Element Research
      Print ISSN
      1634984
      Volume
      154
      Issue
      3
      Pages
      338 - 344
      Language
      English
      Type
      Article
      Item Usage Stats
      148
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      142
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      Abstract
      Placental type 3 iodothyronine deiodinase (D3) potentially protects the fetus from the elevated maternal thyroid hormones. Na+/I- symporter (NIS) is a plasma membrane glycoprotein, which mediates active iodide uptake. Our objectives were to establish the distribution of NIS and D3 gene expressions in the placenta and the amniotic membrane and to investigate the relationship between placental D3 and NIS gene expressions and maternal iodine, selenium, and thyroid hormone status. Thyroid hormones, urinary iodine concentration (UIC), and selenium levels were measured in 49 healthy term pregnant women. NIS and D3 gene expressions were studied with the total mRNA RT-PCR method in tissues from maternal placenta (n = 49), fetal placenta (n = 9), and amniotic membrane (n = 9). NIS and D3 gene expressions were shown in the fetal and maternal sides of the placenta and amniotic membrane. Mean blood selenium level was 66 ± 26.5 μg/l, and median UIC was 143 μg/l. We could not demonstrate any statistically significant relationship of spot UIC and blood selenium with NIS and D3 expression (p > 0.05). Positive correlations were found between NIS and thyroxine-binding globulin (TBG) (r = 0.3, p = 0.042) and between D3 and preoperative glucose levels (r = 0.4, p = 0.006). D3 and NIS genes are expressed in term placenta and amniotic membrane; thus, in addition to placenta, amniotic membrane contributes to regulation of maternofetal iodine and thyroid hormone transmission. Further studies are needed to clarify the relationship between maternal glucose levels and placental D3 expression and between TBG and placental NIS expression. © 2013 Springer Science+Business Media New York.
      Keywords
      Amniotic membrane
      Iodine
      Na+/I- symporter
      Placenta
      Selenium
      Type 3 iodothyronine deiodinase
      glucose
      insulin
      iodine
      liothyronine
      messenger RNA
      prealbumin
      selenium
      sodium iodide symporter
      thyroid hormone
      thyrotropin
      thyroxine
      thyroxine binding globulin
      thyroxine deiodinase
      adult
      amnion
      analytical equipment
      article
      atomic absorption spectrometry
      blood level
      cesarean section
      correlation analysis
      echograph
      echography
      female
      gene expression
      glucose blood level
      human
      immunoradiometric assay
      normal human
      placenta
      preoperative evaluation
      radioimmunoassay
      reverse transcription polymerase chain reaction
      spectrometer
      urinalysis
      volunteer
      Adult
      Amnion
      Female
      Gene Expression Regulation, Developmental
      Gestational Age
      Humans
      Iodide Peroxidase
      Iodine
      Placenta
      Pregnancy
      Reverse Transcriptase Polymerase Chain Reaction
      Selenium
      Symporters
      Thyroid Hormones
      Thyroxine-Binding Globulin
      Permalink
      http://hdl.handle.net/11693/20834
      Published Version (Please cite this version)
      http://dx.doi.org/10.1007/s12011-013-9748-y
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      • Department of Molecular Biology and Genetics 442
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