dc.contributor.advisor | Tekinay, Ayşe Begüm | |
dc.contributor.author | Garip, İmmihan Ceren | |
dc.date.accessioned | 2016-01-08T20:06:47Z | |
dc.date.available | 2016-01-08T20:06:47Z | |
dc.date.issued | 2014 | |
dc.identifier.uri | http://hdl.handle.net/11693/17106 | |
dc.description | Ankara : The Materials Science and Nanotechnology Program and the Graduate School of Engineering and Science of Bilkent University, 2014. | en_US |
dc.description | Thesis (Master's) -- Bilkent University, 2014. | en_US |
dc.description | Includes bibliographical references leaves 76-83. | en_US |
dc.description.abstract | The extracellular matrix (ECM) is crucial for the coordination and regulation of
various cellular processes, including cell adhesion, recruitment, differentiation and
death. ECM components structurally support tissue function and regeneration by
acting as a substrate for cell migration and differentiation. In addition, by facilitating
the fine localization of signals within their structural framework, these components
activate receptors on the cell membrane for the initiation of signal transduction
cascades. As such, cell-matrix interactions and matrix-associated signals are
important for the normal functioning of cells, as well as for natural or artificially
assisted tissue regeneration. In keeping with this ECM-centric approach, we designed
and synthesized peptide amphiphiles with bioactive epitopes to resemble the native
microenvironment of muscle tissue and to examined their potential in the induction
of progenitor cell differentiation into skeletal myotubes and cardiac myocytes. The
formation of skeletal myotubes was promoted through the use of basal laminamimetic
peptide nanofibers inspired by the chemical structures of laminin and
fibronectin, two proteins strongly represented in the skeletal muscle extracellular
matrix. We demonstrated that our basal lamina mimetic peptide nanofiber system
actively interacts with the cells it contains and enhances their differentiation within 3
days. Morphological analysis and immunocytochemical stainings indicated the
formation of differentiated myotubes.We also designed glycosaminoglycan-mimetic peptide amphiphiles to mimic the
glycosaminoglycans found in the myocardium. Glycosaminoglycans have been
reported to play substantial roles in growth factor binding and the induction of
angiogenesis, and their mimicry through peptide amphiphile nanofibers is promising
as a combined approach for generating multifunctional cardiovascular tissue
engineering scaffolds. We demonstrated that peptide nanofibers enhance the
adhesion of cells to the surface and induce cardiac myoblast cells to differentiate into
cardiomyocytes through both gene expression analysis and immunostainings.
In summary, myogenic platforms were developed by programming signal rich
environment from self-assembled peptide nanofibers inspired from the components
of the ECM to induce the differentiation of cells. These bioactive nanofiber systems
serve as promising platforms for muscle tissue engineering applications. | en_US |
dc.description.statementofresponsibility | Garip, İmmihan Ceren | en_US |
dc.format.extent | xv 83 leaves, charts, illustrations | en_US |
dc.language.iso | English | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Peptide nanofibers | en_US |
dc.subject | extracellular matrix | en_US |
dc.subject | biomimetic | en_US |
dc.subject | basal lamina | en_US |
dc.subject | skeletal muscle tissue | en_US |
dc.subject | laminin | en_US |
dc.subject | fibronectin | en_US |
dc.subject | glycosaminoglycan | en_US |
dc.subject | myocardial
regeneration | en_US |
dc.subject.lcc | QP552.E95 G37 2014 | en_US |
dc.subject.lcsh | Extracellular matrix. | en_US |
dc.subject.lcsh | Peptides. | en_US |
dc.subject.lcsh | Biomimetics. | en_US |
dc.title | Programming microenvironmental signals with bioactive peptide amphiphiles for skeletal and cardiac myogenesis | en_US |
dc.type | Thesis | en_US |
dc.department | Graduate Program in Materials Science and Nanotechnology | en_US |
dc.publisher | Bilkent University | en_US |
dc.description.degree | M.S. | en_US |