dc.contributor.advisor | Konu, Özlen | |
dc.contributor.author | Ölmezer, Gizem | |
dc.date.accessioned | 2016-01-08T18:23:51Z | |
dc.date.available | 2016-01-08T18:23:51Z | |
dc.date.issued | 2011 | |
dc.identifier.uri | http://hdl.handle.net/11693/15737 | |
dc.description | Ankara : The Department of Molecular Biology and Genetics and the Graduate School of Engineering and Science of Bilkent University, 2011. | en_US |
dc.description | Thesis (Master's) -- Bilkent University, 2011. | en_US |
dc.description | Includes bibliographical references leaves 93-110. | en_US |
dc.description.abstract | The expression and activity of enzymes taking role in drug metabolism are important
as in the case of phase II glucuronidation enzymes; namely UDPglucuronosyltransferases
(UGTs). Previously, it has been identified that smoking
upregulates the expression of UGT enzymes in oral mucosa. We asked whether
smoking induces UGT1A expression in other tissues and re-analyzed publically
available datasets run with samples from smokers and non-smokers. It was observed
that UGT1A enzymes were overexpressed in several types of epithelial cells of
smokers.
30% of nicotine metabolism is performed by UGT enzymes; however, whether
UGT1A expression is modulated by nicotine, the addictive component of tobacco
smoke, is not known. For this purpose, the expression levels of UGT1A isoforms
were measured using Real-Time PCR in nicotine treated SW620 colorectal cancer
cells. Our findings showed that nicotine’s effect on UGT1A expression was isoform
specific; and the magnitude of modulation differed among isoforms. Furthermore,
the upregulation of UGT1A enzymes could only be observed in serum-deprived
SW620 cells. In summary, nicotine metabolism enzymes are regulated by both
smoking in vivo and nicotine in vitro. Nevertheless, enhanced xenobiotic metabolism
may result in chemoresistance, which is undesirable for cancer patients. Therefore,
before drug therapy cancer patients might be analyzed in terms of their smoking
status and UGT1A expression patterns. | en_US |
dc.description.statementofresponsibility | Ölmezer, Gizem | en_US |
dc.format.extent | xv, 110 leaves, illustrations | en_US |
dc.language.iso | English | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Nicotine | en_US |
dc.subject | Smoking | en_US |
dc.subject | UDP-glucuronosyltransferases (UGTs) | en_US |
dc.subject | Serum Starvation | en_US |
dc.subject | Colon Cancer | en_US |
dc.subject.lcc | QP801.N48 O56 2011 | en_US |
dc.subject.lcsh | Nicotine--Metabolism. | en_US |
dc.subject.lcsh | Nicotine--Physiological effects. | en_US |
dc.subject.lcsh | Colon (Anatomy)--Cancer. | en_US |
dc.subject.lcsh | Enzymes. | en_US |
dc.title | Smoking and nicotine alter UGT1A expression | en_US |
dc.type | Thesis | en_US |
dc.department | Department of Molecular Biology and Genetics | en_US |
dc.publisher | Bilkent University | en_US |
dc.description.degree | M.S. | en_US |