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dc.contributor.advisorTazebay, Uygar H.en_US
dc.contributor.authorErkek, Serapen_US
dc.date.accessioned2016-01-08T18:17:49Z
dc.date.available2016-01-08T18:17:49Z
dc.date.issued2008
dc.identifier.urihttp://hdl.handle.net/11693/15389
dc.descriptionAnkara : The Department of Molecular Biology and Genetics and the Institute of Engineering and Sciences of Bilkent University, 2008.en_US
dc.descriptionThesis (Master's) -- Bilkent University, 2008.en_US
dc.descriptionIncludes bibliographical references leaves 47-52.en_US
dc.description.abstractHANEIN-1 was first identified in a study investigating 3’ transcriptional regulatory elements of the Na+ /I- symporter gene. The protein is highly conserved among eukaryotes and bears no domain similarities with any known proteins. In databases, it is described as coiled coil containing-124 hypothetical protein and predicted to encode a 223 amino acid-protein. In this project, our aim was to characterize this highly conserved protein by using several bioinformatics and biochemical methodologies. Sequence similarity search analysis showed that it had around 75% identity in mammals, 50% identity with insects and nematodes. Expressional analysis revealed that HANEIN-1 was expressed in all tissues ubiquitously with a remarkable expression status in skeletal muscle. Beside providing information about expression status of HANEIN-1, northern blotting showed that HANEIN-1 transcript size was approximately 1000 bp. Regarding protein level expression, western blotting revealed that HANEIN-1 encoded a 33 kDa protein and protein stability was affected in a different way upon labeling with Flag epitope at N-ter and C-ter. Yeast double hybrid screening performed in our laboratory showed that HANEIN-1 interacted with RASGEF1B, which was a guanine nucleotide exchange factor not fully characterized. Expressional analysis displayed that RASGEF1B expression profile inversely correlated with HANEIN-1. Finally, serine-scanning mutagenesis analysis showed that site-directed mutagenesis of serine at position 194 significantly affected the stability of the protein.en_US
dc.description.statementofresponsibilityErkek, Serapen_US
dc.format.extentxi, 52 leaves, illustrationsen_US
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccQP551 .E74 2008en_US
dc.subject.lcshProteins--Analysis.en_US
dc.subject.lcshEukaryetic cells.en_US
dc.subject.lcshGenetic transcription.en_US
dc.titleHanein-1, a novel conserved eukaryotic protein ubiquitously expressed in human tissuesen_US
dc.typeThesisen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.publisherBilkent Universityen_US
dc.description.degreeM.S.en_US


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