dc.contributor.advisor | Akçalı, K. Can | |
dc.contributor.author | Tokcaer Keskin, Zeynep | |
dc.date.accessioned | 2016-01-08T18:13:34Z | |
dc.date.available | 2016-01-08T18:13:34Z | |
dc.date.issued | 2010 | |
dc.identifier.uri | http://hdl.handle.net/11693/15103 | |
dc.description | Ankara : The Department of Molecular Biology and Genetics and the Institute of Engineering and Science of Bilkent University, 2010. | en_US |
dc.description | Thesis (Ph. D.) -- Bilkent University, 2010. | en_US |
dc.description | Includes bibliographical references leaves 73-106. | en_US |
dc.description.abstract | tem cell research evolved as a new hope and has gained tremendous interest in
the last two decades to develop new strategies for many of debilitating diseases.
Mesenchymal Stem Cells (MSCs) are multipotent cells capable of self-renewal
and differentiating into multiple lineages such as osteocytes, adipocytes,
chondrocytes, myoblasts, and hepatocytes. MSCs can migrate to the injured tissue
and have immunomodulatory effects. Due to these features, MSCs have high
therapeutic value in tissue engineering and regenerative medicine. In this thesis,
our aim was to investigate the further contribution of the MSCs in different
cellular therapies. We used two approaches to accomplish our aim. First, we
investigated the possibility of obtaining functional cardiomyocytes from rat MSC
within a shorter time period by determining the induction timing of
cardiomyocyte differentiation of MSCs. Our data revealed that it is possible to get
functional cardiomyocytes from in vitro MSC culture in a shorter time period than
previously achieved. This reduction in time may provide emergency cases with
access to cell-based therapies that may have previously been unavailable. In the
second part of this thesis, we examined in vivo and in vitro effects of a telomerase
antagonist, imetelstat (GRN163L) on MSCs. Telomerase activity is essential for
the continued growth and survival of malignant cells, therefore inhibition of this
activity presents an attractive target for anti-cancer therapy. MSCs also show
telomerase activity in maintaining their self-renewal; therefore the effects of
telomerase inhibitors on MSCs may be an issue of concern. Our results showed
that inhibiting the telomerase activity does not interfere with the self-renewal and
differentiation of MSCs under short term in vitro culture conditions. | en_US |
dc.description.statementofresponsibility | Keskin, Zeynep Tokcaer | en_US |
dc.format.extent | xiii, 110, [34] leaves, illustrations | en_US |
dc.language.iso | English | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject.lcc | QH588.S83 K47 2010 | en_US |
dc.subject.lcsh | Mesenchymal stem cells. | en_US |
dc.subject.lcsh | Stem cells--Therapeutic use. | en_US |
dc.subject.lcsh | Immunogenetics. | en_US |
dc.title | Contribution of mesenchymal stem cells in cell based therapies | en_US |
dc.type | Thesis | en_US |
dc.department | Department of Molecular Biology and Genetics | en_US |
dc.publisher | Bilkent University | en_US |
dc.description.degree | Ph.D. | en_US |
dc.identifier.itemid | B122783 | |