Nanostructured materials for biological imaging and chemical sensing
Author
Yıldırım, Adem
Advisor
Bayındır, Mehmet
Date
2014-11Publisher
Bilkent University
Language
English
Type
ThesisItem Usage Stats
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Abstract
In the recent years, the design and synthesis of fluorescent nanoparticles for
biological and chemical sensing applications have received considerable attention due
to the excellent photostability and emission intensity of fluorescent nanoparticles and
the intrinsic sensitivity of fluorescence based methods. Although considerable
progress has been made in their synthesis, there is still need for low-cost and high
throughput methods for their widespread utilization in biological and chemical sensing
applications. In addition, studies regarding their biocompatibility are necessary to
identify the toxicological potential of these nanomaterials.
In this context, this thesis seeks new methods for multifunctional fluorescent
nanoparticle synthesis and investigates their interactions with living organisms. In
addition, it reports the applications of the fluorescent nanomaterials in biological
imaging, therapy and chemical sensing applications.
First, we report a self-assembly method to prepare PEGylated or peptide
functionalized mesoporous silica nanoparticles (MSNs) for cell labeling and drug
delivery applications. The good cyto- and blood- compatibility of the functionalized
nanoparticles were demonstrated. Next, we demonstrated a surfactant assisted method
to synthesize ultrabright silica nanoparticles and studied their in vitro
v
cytocompatibility with several cell lines. We demonstrated the applications of
ultrabright particles in cell labeling, chemo and photodynamic therapy and trace
explosive sensing. Then, we discuss a template-free method (porosity difference based
selective dissolution strategy) to prepare self-luminescent mesoporous hollow silica
nanoparticles with tailored shapes. In addition, we studied the surface effects on blood
compatibility of nanoparticles in detail using the MSNs possessing different surface
functional groups (ionic, polar, neutral, and hydrophobic). Finally, we investigated the
optical properties of polydopamine nanoparticles and showed that fluorescence of asprepared
polydopamine nanoparticles can be used for sensitive and selective detection
of the dopamine neurotransmitter.
Keywords
Fluorescent nanoparticlesMesoporous silica
Polydopamine
Blood compatibility
Cytotoxicity
Fluorescent imaging
Chemotherapy
Photodynamic therapy
Explosive sensing
Neurotransmitter sensing
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