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dc.contributor.authorUzun, S. D.en_US
dc.contributor.authorKayaci, F.en_US
dc.contributor.authorUyar, T.en_US
dc.contributor.authorTimur, S.en_US
dc.contributor.authorToppare, L.en_US
dc.date.accessioned2015-07-28T12:03:01Z
dc.date.available2015-07-28T12:03:01Z
dc.date.issued2014-03-24en_US
dc.identifier.issn1944-8244
dc.identifier.urihttp://hdl.handle.net/11693/12786
dc.description.abstractThe combination of nanomaterials and conducting polymers attracted remarkable attention for development of new immobilization matrices for enzymes. Hereby, an efficient surface design was investigated by modifying the graphite rod electrode surfaces with one-step electrospun nylon 6,6 nanofibers or 4% (w/w) multiwalled carbon nanotubes (MWCNTs) incorporating nylon 6,6 nanofibers (nylon 6,6/4MWCNT). High-resolution transmission electron microscopy study confirmed the successful incorporation of the MWCNTs into the nanofiber matrix for nylon 6,6/4MWCNT sample. Then, these nanofibrous surfaces were coated with a conducting polymer, (poly-4-(4,7-di(thiophen-2-yl)-1H-benzo[d]imidazol-2-yl) benzaldehyde) (PBIBA) to obtain a high electroactive surface area as new functional immobilization matrices. Due to the free aldehyde groups of the polymeric structures, a model enzyme, glucose oxidase was efficiently immobilized to the modified surfaces via covalent binding. Scanning electron microscope images confirmed that the nanofibrous structures were protected after the electrodeposition step of PBIBA and a high amount of protein attachment was successfully achieved by the help of high surface to volume ratio of electroactive nanofiber matrices. The biosensors were characterized in terms of their operational and storage stabilities and kinetic parameters (K mapp and Imax). The resulting novel glucose biosensors revealed good stability and promising Imax values (10.03 and 16.67 μA for nylon 6,6/PBIBA and nylon 6,6/4MWCNT/PBIBA modified biosensors, respectively) and long shelf life (32 and 44 days for nylon 6,6/PBIBA and nylon 6,6/4MWCNT/PBIBA modified biosensors, respectively). Finally, the biosensor was tested on beverages for glucose detection. © 2014 American Chemical Society.en_US
dc.language.isoEnglishen_US
dc.source.titleACS Applied Materials and Interfacesen_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/am5005927en_US
dc.subjectElectrospinningen_US
dc.subjectNylon 6,6 nanofibersen_US
dc.subjectMWCNTen_US
dc.subjectConducting polymeren_US
dc.subjectCovalent immobilizationen_US
dc.subjectGlucose oxidaseen_US
dc.subjectAmperometric biosensoren_US
dc.titleBioactive surface design based on functional composite electrospun nanofibers for biomolecule immobilization and biosensor applicationsen_US
dc.typeArticleen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.citation.spage5235en_US
dc.citation.epage5243en_US
dc.citation.volumeNumber6en_US
dc.citation.issueNumber7en_US
dc.instituteInstitute of Materials Science and Nanotechnologyen_US
dc.identifier.doi10.1021/am5005927en_US
dc.publisherAmerican Chemical Societyen_US


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