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dc.contributor.authorGülsuner, Hilal Unalen_US
dc.contributor.authorGengec, N. A.en_US
dc.contributor.authorKılınç, Muraten_US
dc.contributor.authorErbil, H. Y.en_US
dc.contributor.authorTekinay, Ayse B.en_US
dc.date.accessioned2015-07-28T12:02:56Z
dc.date.available2015-07-28T12:02:56Z
dc.date.issued2015en_US
dc.identifier.issn1549-3296
dc.identifier.urihttp://hdl.handle.net/11693/12775
dc.description.abstractThe instability of implants after placement inside the body is one of the main obstacles to clinically succeed in periodontal and orthopedic applications. Adherence of fibroblasts instead of osteoblasts to implant surfaces usually results in formation of scar tissue and loss of the implant. Thus, selective bioadhesivity of osteoblasts is a desired characteristic for implant materials. In this study, we developed osteoselective and biofriendly polymeric thin films fabricated with a simple phase separation method using either homopolymers or various blends of homopolymers and copolymers. As adhesive and proliferative features of cells are highly dependent on the physicochemical properties of the surfaces, substrates with distinct chemical heterogeneity, wettability, and surface topography were developed and assessed for their osteoselective characteristics. Surface characterizations of the fabricated polymer thin films were performed with optical microscopy and SEM, their wettabilities were determined by contact angle measurements, and their surface roughness was measured by profilometry. Long-term adhesion behaviors of cells to polymer thin films were determined by F-actin staining of Saos-2 osteoblasts, and human gingival fibroblasts, HGFs, and their morphologies were observed by SEM imaging. The biocompatibility of the surfaces was also examined through cell viability assay. Our results showed that heterogeneous polypropylene polyethylene/polystyrene surfaces can govern Saos-2 and HGF attachment and organization. Selective adhesion of Saos-2 osteoblasts and inhibited adhesion of HGF cells were achieved on micro-structured and hydrophobic surfaces. This work paves the way for better control of cellular behaviors for adjustment of cell material interactions.en_US
dc.language.isoEnglishen_US
dc.source.titleJournal of Biomedical Materials Research Part Aen_US
dc.relation.isversionofhttp://dx.doi.org/10.1002/jbm.a.35164en_US
dc.subjectPolymer thin filmen_US
dc.subjectSaos-2en_US
dc.subjectMicro-structureen_US
dc.subjectPhase separationen_US
dc.subjectOsteoselectionen_US
dc.subjectHuman gingival fibroblasten_US
dc.titleOsteoselection supported by phase separated polymer blend filmsen_US
dc.typeArticleen_US
dc.departmentInstitute of Materials Science and Nanotechnology (UNAM)en_US
dc.departmentNanotechnology Research Center (NANOTAM)en_US
dc.departmentAysel Sabuncu Brain Research Center (BAM)en_US
dc.citation.spage154en_US
dc.citation.epage161en_US
dc.citation.volumeNumber103en_US
dc.citation.issueNumber1en_US
dc.identifier.doi10.1002/jbm.a.35164en_US
dc.publisherWileyen_US
dc.contributor.bilkentauthorGülsuner, Hilal Unal
dc.contributor.bilkentauthorKılınç, Murat
dc.contributor.bilkentauthorTekinay, Ayse B.


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