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dc.contributor.authorSackesen, C.en_US
dc.contributor.authorAkdis, M.en_US
dc.contributor.authorSoyer, O.en_US
dc.contributor.authorZumkehr, J.en_US
dc.contributor.authorRuckert, B.en_US
dc.contributor.authorKalayci, O.en_US
dc.contributor.authorAlkan, S. S.en_US
dc.contributor.authorGursel, I.en_US
dc.contributor.authorAkdis, C. A.en_US
dc.contributor.authorStanic, B.en_US
dc.contributor.authorVeen, W. V.en_US
dc.date.accessioned2015-07-28T12:02:02Z
dc.date.available2015-07-28T12:02:02Z
dc.date.issued2013en_US
dc.identifier.issn0105-4538
dc.identifier.urihttp://hdl.handle.net/11693/12572
dc.description.abstractBackground The fine balance of immunoglobulins (Ig) E, IgG1, IgG4 and IgA in healthy production is maintained by the interaction of B cells with adaptive and innate immune response. The regulation of toll-like receptors (TLRs)-driven innate and adaptive immune effector B-cell response and the role of mammalian telomeric TTAGGG repeat elements represent an important research area. Methods Human PBMC and purified naive and memory B cells were stimulated with specific ligands for TLR2, TLR3, TLR4, TLR5, TLR7, TLR8 and TLR9 in the presence or absence of telomeric oligonucleotides. B-cell proliferation, differentiation and antibody production were determined. Results TLR9 ligand directly activates naive and memory B cells, whereas TLR7 can stimulate them in the presence of plasmacytoid dendritic cells. Human B cells proliferate and turn into antibody-secreting cells in response to TLR3, TLR7 and TLR9, but not to TLR2, TLR4, TLR5 and TLR8 ligands. Stimulation of B cells with intracellular TLR3, TLR7 and TLR9 induced an activation cascade leading to memory B-cell generation and particularly IgG1, but also IgA, IgG4 and very low levels of IgE production. Mammalian telomeric oligodeoxynucleotide (ODN) significantly inhibited all features of TLR ligand-induced events in B cells including B-cell proliferation, IgE, IgG1, IgG4, IgA production, class switch recombination, plasma cell differentiation induced by TLR3, TLR7 and TLR9 ligands. Conclusion B cells require specific TLR stimulation, T-cell and plasmacytoid dendritic cell help for distinct activation and Ig production profiles. Host-derived telomeric ODN suppress B-cell activation and antibody production demonstrating a natural mechanism for the control of overexuberant B-cell activation, antibody production and generation of memory. © 2013 John Wiley & Sons A/S. Published by Blackwell Publishing Ltd.en_US
dc.language.isoEnglishen_US
dc.source.titleAllergyen_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/all.12133en_US
dc.subjectB Cellen_US
dc.subjectImmunoglobulins Aen_US
dc.subjectG1en_US
dc.subjectG4en_US
dc.subjectEen_US
dc.subjectPlasmacytoid Dendritic Cellen_US
dc.subjectTelomeric Oligodeoxynucleotideen_US
dc.subjectToll-like Receptor Liganden_US
dc.titleSuppression of B cell activation and IgE, IgG1 and IgG4 production by mammalian telomeric oligonucleotidesen_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.citation.spage593en_US
dc.citation.epage603en_US
dc.citation.volumeNumber68en_US
dc.citation.issueNumber5en_US
dc.identifier.doi10.1111/all.12133en_US
dc.publisherWileyen_US
dc.identifier.eissn0105-4538


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