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      Label-free nanometer-resolution imaging of biological architectures through surface enhanced raman scattering

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      Author
      Ayas, Sencer
      Çınar, Göksu
      Özkan, Alper Devrim
      Soran, Zeliha
      Ekiz, Oner
      Kocaay, Deniz
      Tomak, A.
      Toren, Pelin
      Kaya, Yasin
      Tunc, I.
      Zareie, H.
      Tekinay, T.
      Tekinay, Ayse Begum
      Güler, Mustafa O.
      Dana, Aykutlu
      Date
      2013
      Source Title
      Scientific Reports
      Print ISSN
      2045-2322
      Publisher
      Nature Publishing Group
      Volume
      3
      Pages
      2624 - 2624
      Language
      English
      Type
      Article
      Item Usage Stats
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      Abstract
      Label free imaging of the chemical environment of biological specimens would readily bridge the supramolecular and the cellular scales, if a chemical fingerprint technique such as Raman scattering can be coupled with super resolution imaging. We demonstrate the possibility of label-free super-resolution Raman imaging, by applying stochastic reconstruction to temporal fluctuations of the surface enhanced Raman scattering (SERS) signal which originate from biomolecular layers on large-area plasmonic surfaces with a high and uniform hot-spot density (> 10(11)/cm(2), 20 to 35 nm spacing). A resolution of 20 nm is demonstrated in reconstructed images of self-assembled peptide network and fibrilated lamellipodia of cardiomyocytes. Blink rate density is observed to be proportional to the excitation intensity and at high excitation densities (> 10 kW/cm(2)) blinking is accompanied by molecular breakdown. However, at low powers, simultaneous Raman measurements show that SERS can provide sufficient blink rates required for image reconstruction without completely damaging the chemical structure.
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      http://hdl.handle.net/11693/12174
      Published Version (Please cite this version)
      http://dx.doi.org/10.1038/srep02624
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      • Aysel Sabuncu Brain Research Center (BAM) 196
      • Department of Electrical and Electronics Engineering 3597
      • Institute of Materials Science and Nanotechnology (UNAM) 1831
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