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      • Department of Molecular Biology and Genetics
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      Characterization of differential expression patterns of the extracellular purinergic enzymes in colorectal cancer

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      Author(s)
      Göktuna, Serkan
      Date
      2022-10-15
      Source Title
      Trakya University Journal of Natural Sciences
      Electronic ISSN
      2528-9691
      Publisher
      Trakya University
      Volume
      23
      Issue
      2
      Pages
      177 - 184
      Language
      English
      Type
      Article
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      Abstract
      The aim of this study is to characterize tumor cell specific expression of purinergic ecto-enzymes CD39 and CD73, and to associate prognostic significance of these expression patterns in colorectal cancer (CRC) patients. Protein and gene expression of the target genes in various CRC cell lines were assessed via Western Blot (WB) analysis and Real Time PCR (RT-PCR). Additionally, tumor vs stromal cell expression of the target genes was analyzed from publicly available patient expression datasets. Finally, the correlation between CD39 and CD73 expression with patient prognosis was analyzed via The Cancer Genome Atlas (TCGA) datasets. In CRC cell lines, CD39 was found to be not expressed at all while CD73 was expressed extensively in most cell lines via WB and RT-PCR analyses. Patient microarray expression data confirmed the results from CRC cell lines that CD39 expression was very low in epithelial/tumor cells relative to other stromal cell types yet CD73 was expressed abundantly in every cell type within patient tumor samples. Interestingly, CD39 expression in patient tumors was correlated with favorable prognosis while CD73 expression was associated with worse prognosis. Although CD39 and CD73 are related enzymes involved in extracellular purinergic signaling, their expression patterns in tumor cells and prognostic effects in patients show opposing outcomes. Therefore, better insights in understanding the functional involvement of purinergic ecto-enzymes in colorectal tumor development is needed via further mechanistic studies.
      Keywords
      Cancer
      Adenosine
      CD39
      CD73
      Prognosis
      Permalink
      http://hdl.handle.net/11693/111900
      Published Version (Please cite this version)
      10.23902/trkjnat.1139062
      Collections
      • Department of Molecular Biology and Genetics 542
      • Institute of Materials Science and Nanotechnology (UNAM) 2258
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