Browsing by Subject "Allergy"
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Item Open Access Contribution of plasma microparticles to the clinical manifestation of allergic diseases(Turkish Society of Immunology, 2012-04) Kahraman, Tamer; Erkoçoğlu, M.; Azkur, D.; Kocabaş, C. N.; Gürsel, İhsanMicroparticles (MPs) are nanovesicles secreted from wide variety of cells. They have role in cellular communications in addition to their physiological roles in several diseases. In this study, we investigated roles of MPs in allergic diseases. Method Peripheral blood from 43 asthma, 15 atopic dermatitis and 13 healthy subjects were collected. MPs were isolated via differential centrifugation and subjected to Annexin-V staining together with different cell specific surface markers (CD9, CD14, CD42a, CD69 and CD105) and analyzed by FACS. Internalization of MPs by PBMCs were assessed by SP-DiOC staining. Results FACS analysis demonstrated that number of MPs in ml plasma was 8,3 ± 1,5 x105, 3,2 ± 1,8 x105, 1,9 ± 0,8 x105, and 3,2 ± 2,2 x105 in healthy, atopic dermatitis, asthma controlled and asthma attack subjects, respectively.Item Open Access Distinct regulation of tonsillar immune response in virus infection(Wiley-Blackwell Publishing Ltd., 2014) Jartti, T.; Palomares, O.; Waris, M.; Tastan, O.; Nieminen, R.; Puhakka, T.; Rückert, B.; Aab, A.; Vuorinen, T.; Allander, T.; Vahlberg, T.; Ruuskanen, O.; Akdis, M.; Akdis, C. A.Background: The relationships between tonsillar immune responses, and viral infection and allergy are incompletely known. Objective To study intratonsillar/nasopharyngeal virus detections and in vivo expressions of T-cell- and innate immune response-specific cytokines, transcription factors, and type I/II/III interferons in human tonsils. Methods: Palatine tonsil samples were obtained from 143 elective tonsillectomy patients. Adenovirus, bocavirus-1, coronavirus, enteroviruses, influenza virus, metapneumovirus, parainfluenza virus, rhinovirus, and respiratory syncytial virus were detected using PCR. The mRNA expression levels of IFN-α, IFN-β, IFN-γ, IL-10, IL-13, IL-17, IL-28, IL-29, IL-37, TGF-β, FOXP3, GATA3, RORC2, and Tbet were directly analyzed by quantitative RT-PCR. Results Fifty percentage of subjects reported allergy, 59% had ≥1 nasopharyngeal viruses, and 24% had ≥1 intratonsillar viruses. Tonsillar virus detection showed a strong negative association with age; especially rhinovirus or parainfluenza virus detection showed positive association with IFN-γ and Tbet expressions. IL-37 expression was positively associated with atopic dermatitis, whereas IFN-α, IL-13, IL-28, and Tbet expressions were negatively associated with allergic diseases. Network analyses demonstrated strongly polarized clusters of immune regulatory (IL-10, IL-17, TGF-β, FOXP3, GATA3, RORC2, Tbet) and antiviral (IFN-α, IFN-β, IL-28, IL-29) genes. These two clusters became more distinctive in the presence of viral infection or allergy. A negative correlation between antiviral cytokines and IL-10, IL-17, IL-37, FOXP3, and RORC2 was observed only in the presence of viruses, and interestingly, IL-13 strongly correlated with antiviral cytokines. Conclusions: Tonsillar cytokine expression is closely related to existing viral infections, age, and allergic illnesses and shows distinct clusters between antiviral and immune regulatory genes. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.