Eroğlu, C.Yıldız, E.Öztürk, M.Pınarbaşı, E.2016-02-082016-02-0820000001-723Xhttp://hdl.handle.net/11693/24999In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3(rd) generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.EnglishCloningCore protein geneELISAExpressionHepatitis C virusNucleotide sequencingPurificationRT-PCRComplementary DNACore proteinHepatitis C antibodyRNASerologic TestsViral Core ProteinsEscherichia coliArticle1336-2305