Beksac, M.Ma, M.Akyerli, C.DerDanielian, M.Zhang, L.Liu, J.Arat, M.Konuk, N.Koc, H.Ozcelik, T.Vescio, R.Berenson, J. R.2016-02-082016-02-0820010887-6924http://hdl.handle.net/11693/24811In order to investigate the frequency of HHV-8 in MM patients from another geographic location, we obtained fresh bone marrow (BM) biopsies from Turkish patients with MM (n = 21), monoclonal gammopathy of undetermined significance (MGUS) (n = 2), plasmacytoma (n = 1) with BM plasma cell infiltration, various hematological disorders (n = 6), and five healthy Turkish controls. The frequency of HHV-8 was analyzed by polymerase chain reaction (PCR) in two independent laboratories in the USA and in Turkey. Using fresh BM biopsies, 17/21 MM patients were positive for HHV-8 whereas all five healthy controls, and six patients with other hematological disorders were negative. Two patients with MGUS, and one patient with a solitary plasmacytoma were also negative. The data from the two laboratories were completely concordant. Also using primer pairs for v IRF and v IL-8R confirmed the results observed with the KS330233 primers. Furthermore, sequence analysis demonstrated a C3 strain pattern in the ORF26 region which was also found in MM patients from the US. Thus, HHV-8 is present in the majority of Turkish MM patients, and the absence of the virus in healthy controls further supports its role in the pathogenesis of MM.EnglishFrequency and sequenceHHV-8Multiple myelomaTurkish patientsImmunoglobulin heavy chainImmunoglobulin receptorInterleukin 8 receptorPlasma proteinPrimer DNABasement membraneBone marrow biopsyCell infiltrationClinical articleControlled studyFrequency analysisGeographic distributionHematologic diseaseHuman herpesvirus 8Human tissueLaboratory testMonoclonal immunoglobulinemiaMultiple myelomaNonhumanOpen reading framePathogenesisPlasma cellPlasmacytomaPolymerase chain reactionPriority journalSequence analysisTurkey (republic)United StatesVirus detectionVirus strainBiopsyBone MarrowHerpesviridae infectionsHerpesvirus 8, humanHumansMultiple MyelomaTurkeyArticle10.1038/sj.leu.2402190