Soran, Z.Aydin, R.S.T.Gumusderelioglu, M.2016-02-082016-02-0820120265-2048http://hdl.handle.net/11693/21201The aim of this study is to develop an effective growth factor releasing scaffold-microsphere system for promoting periodontal tissue engineering. Bone morphogenetic protein-6 (BMP-6)-loaded alginate microspheres in narrow size distribution were produced by optimising electrospraying conditions. The addition of these microspheres to chitosan gels produced a novel scaffold in which not only the pore sizes and interconnectivity were preserved, but also a controlled release vehicle was generated. Loading capacity was adjusted as 50ng or 100ng BMP-6 for each scaffold and the controlled release behaviour of BMP-6 from chitosan scaffolds was observed during seven days. Cell culture studies were carried out with rat mesenchymal stem cells derived from bone marrow in three groups; chitosan scaffolds, chitosan scaffolds containing BMP-6-loaded alginate microspheres and chitosan scaffolds with free BMP-6 in culture medium. Results showed that controlled delivery of BMP-6 from alginate microspheres has a significant effect on osteogenic differentiation. © 2012 Informa UK Ltd All rights reserved.EnglishAlginate microspheresBMP-6Chitosan scaffoldMesenchymal stem cellsPeriodontal tissue engineeringalginic acidbone morphogenetic protein 6chitosanmicrosphereanimal cellarticlebone marrowcell culturecell differentiationcontrolled release formulationculture mediumdrug releaseelectrospraymesenchymal stem cellnonhumanperiodontiumrattissue engineeringAlginatesAnimalsBone Morphogenetic Protein 6ChitosanCulture MediaGlucuronic AcidHexuronic AcidsMesenchymal Stromal CellsMicroscopy, Electron, ScanningMicrospheresPeriodontiumRatsTissue EngineeringArticle10.3109/02652048.2012.686531