Zhang, Y.Kyle J.R.Penchev, M.Yazdanpanah V.Yu J.Li, Y.Yang, M.Budak G.Özbay, EkmelOzkan, M.Ozkan, C.S.2016-02-082016-02-08201221911630http://hdl.handle.net/11693/21210Cellular uptake behavior of iron oxide nanoparticles is investigated using a transmission near-field scanning optical microscopy (NSOM) without the need of fluorescent labeling. By using the transmission NSOM system, we could simultaneously explore the near-field optical analysis of the cell interior and record the topographic information of the cell surface. The cell endocytosis of iron oxide nanoparticles by normal breast MCF10A cells is first studied by this transmission NSOM system, and this dual functional nanoscale-resolution microscopy shows the capability of mapping the spatial localization of nanoparticles in/outside cell surface without the need of fluorescence labeling. Nanoscale optical signature patterns for iron oxide nanoparticle-loaded vesicles inside the cells were observed and analyzed. © Springer Science+Business Media, LLC 2012.EnglishAFMEndocytosisIron oxideMCF10ANanoparticleNSOMAFMEndocytosisFluorescence labelingFluorescent labelingIron oxide nanoparticleMCF10ASpatial localizationTopographic informationCell membranesCytologyFluorescenceIron oxidesMetal nanoparticlesMolecular biologyNanoparticlesNanotechnologyNear field scanning optical microscopyfluorescent dyeiron oxidenanoparticlearticlecontrolled studydetoxificationdrug delivery systemdrug uptakeendocytosisfluorescencehumanhuman cellkineticslight scatteringnuclear magnetic resonance imagingpolarimetryscanning electron microscopyscanning near field optical microscopytissue repairtopographyzeta potentialArticle10.1007/s12668-012-0043-8