Mokdad-Gargouri, R.Belhadj, K.Gargouri, A.2016-02-082016-02-0820010305-1048http://hdl.handle.net/11693/24887We have expressed human p53 cDNA in the yeast Saccharomyces cerevisiae and shown that the level of production and the lenght of the p53 protein depends on the presence of untranslated mRNA regions (UTRs). The expression of the ORF alone leads to a p53 protein of correct size (53 kDa) that accumulates to high levels, concomitantly with the presence of a small amount of a p40 protein (40 kDa). However, when either the entire 5′-UTR and a part of the 3′- or 5′-UTR alone is used, this leads to the production of small amounts of the 40 kDa truncated form only. The p40 protein corresponds to a truncated form of p53 at the C-terminal extremity since it reacts only with a monoclonal antibody recognising the N-terminal epitope. This effect on the amount and lenght of p53 protein had no correlation at the mRNA level, suggesting that translational control probably occurs through the 5′-UTR. We propose a model of structural interaction between this UTR and a part of the ORF mRNA for the regulation of p53 expression in this heterologous context.Englishcomplementary DNAepitopemessenger RNAmonoclonal antibodyprotein p40protein p533' untranslated region5' untranslated regionamino terminal sequencearticlecarboxy terminal sequencecontrolled studycorrelation functiongene structurehumannonhumanopen reading framepriority journalprotein expressionprotein synthesisSaccharomyces cerevisiaetranslation regulationtumor suppressor geneyeast3' Untranslated Regions5' Untranslated RegionsBlotting, NorthernBlotting, WesternCell DivisionDNA, ComplementaryGene Expression RegulationHumansOpen Reading FramesPlasmidsProtein BiosynthesisRNA, MessengerSaccharomyces cerevisiaeSequence DeletionTranscription, GeneticTumor Suppressor Protein p53Saccharomyces cerevisiaeArticle