Köksaldı, İlkay ÇisilKöse, SılaAhan, Recep ErdemHacıosmanoğlu, NedimŞahin Kehribar, EbruGüngen, Murat AlpBaştuğ, A.Dinç, B.Bodur, H.Özkul, A.Şeker, Urartu Özgür Şafak2022-02-182022-02-182021-06-300003-2700http://hdl.handle.net/11693/77502SARS-CoV-2 is a human pathogen and the main cause of COVID-19 disease, announced as a global pandemic by the World Health Organization. COVID-19 is characterized by severe conditions, and early diagnosis can make dramatic changes for both personal and public health. Low-cost, easy-to-use diagnostic capabilities can have a very critical role in controlling the transmission of the disease. Here, we are reporting a state-of-the-art diagnostic tool developed with an in vitro synthetic biology approach by employing engineered de novo riboregulators. Our design coupled with a home-made point-of-care device can detect and report the presence of SARS-CoV-2-specific genes. The presence of SARS-CoV-2-related genes triggers the translation of sfGFP mRNAs, resulting in a green fluorescence output. The approach proposed here has the potential of being a game changer in SARS-CoV-2 diagnostics by providing an easy-to-run, low-cost diagnostic capability.EnglishSARS-CoV-2SensorsGeneticsTesting and assessmentGenomicsArticle10.1021/acs.analchem.1c008861520-6882