Surface-modified bacterial nanofibrillar PHB scaffolds for bladder tissue repair

Karahaliloǧlu, Z.Demirbilek, M.Şam, M.Saǧlam, N.Mizrak, A. K.Denkbaş, E. B.2018-04-122018-04-1220162169-1401http://hdl.handle.net/11693/36575The aim of the study is in vitro investigation of the feasibility of surface-modified bacterial nanofibrous poly [(R)-3-hydroxybutyrate] (PHB) graft for bladder reconstruction. In this study, the surface of electrospun bacterial PHB was modified with PEG- or EDA via radio frequency glow discharge method. After plasma modification, contact angle of EDA-modified PHB scaffolds decreased from 110 ï¿½ 1.50 to 23 ï¿½ 0.5 degree. Interestingly, less calcium oxalate stone deposition was observed on modified PHB scaffolds compared to that of non-modified group. Results of this study show that surface-modified scaffolds not only inhibited calcium oxalate growth but also enhanced the uroepithelial cell viability and proliferation.EnglishBladder reconstructionCalcium oxalate stonesNanotexturePoly [(R)-3-hydroxybutyrate] (PHB)Tissue engineeringCalciumGlow dischargesOxalic acidTissueTissue engineeringBladder reconstructionCalcium oxalatesNano-texturePlasma modificationsPoly[-3-hydroxybutyrate]Radio frequency glow dischargeSurface-modifiedUroepithelial cellsScaffolds (biology)3 hydroxybutyric acidCalciumCalcium oxalateMolecular scaffoldPoly [(r) 3 hydroxybutyrate]Unclassified drugBiomaterialEthylenediamineEthylenediamine derivativeHydroxybutyric acidMacrogol derivativeAnimal cellArticleCalcium oxalate stoneCell proliferationCell viabilityChemical compositionContact angleCrystallizationElectrospinningFrequencyIn vitro studyNonhumanSurface propertyTissue repairAnimalBladderCell lineCell survivalChemistryCupriavidus necatorDrug effectsEpithelium cellIsolation and purificationMetabolismMouseNephrolithiasisPathologyPolymerizationRegenerationTissue engineeringAnimalsBiocompatible materialsCalcium oxalateCell lineCell proliferationCell survivalCupriavidus necatorEpithelial cellsEthylenediaminesHydroxybutyratesKidney calculiMicePolyethylene glycolsPolymerizationRegenerationTissue engineeringUrinary bladderSurface-modified bacterial nanofibrillar PHB scaffolds for bladder tissue repairArticle10.3109/21691401.2014.9130532169-141X