Onen-Bayram, F. E.Durmaz, I.Scherman, D.Herscovici, J.Cetin Atalay, R.2016-02-082016-02-0820120968-0896http://hdl.handle.net/11693/21334The forward chemogenomics strategy allowed us to identify a potent cytotoxic thiazolidine compound as an apoptosis-inducing agent. Chemical structures were designed around a thiazolidine ring, a structure already noted for its anticancer properties. Initially, we evaluated these novel compounds on liver, breast, colon and endometrial cancer cell lines. The compound 3 (ALC67) showed the strongest cytotoxic activity (IC50 ∼5 μM). Cell cycle analysis with ALC67 on liver cells revealed SubG1/G1 arrest bearing apoptosis. Furthermore we demonstrated that cytotoxicity of this compound was due to the activation of caspase-9 involved apoptotic pathway, which is death receptor independent. © 2012 Elsevier Ltd. All rights reserved.EnglishApoptosisCancerCaspase-9CytotoxicTerminal alkyneThiazolidineArticle10.1016/j.bmc.2012.07.016