Gumuscu, B.Tekinay, T.2016-02-082016-02-0820130964-8305http://hdl.handle.net/11693/20723In this environmental-sample based study, rapid microbial-mediated degradation of 2,4,6-trinitrotoluene (TNT) contaminated soils is demonstrated by a novel strain, Achromobacter spanius STE 11. Complete removal of 100mgL-1 TNT is achieved within only 20h under aerobic conditions by the isolate. In thisbio-conversion process, TNT is transformed to 2,4-dinitrotoluene (7mgL-1), 2,6-dinitrotoluene (3mgL-1), 4-aminodinitrotoluene (49mgL-1) and 2-aminodinitrotoluene (16mgL-1) as the key metabolites. A. spanius STE 11 has the ability to denitrate TNT in aerobic conditions as suggested by the dinitrotoluene and NO3 productions during the growth period. Elemental analysis results indicate that 24.77mgL-1 nitrogen from TNT was accumulated in the cell biomass, showing that STE 11 can use TNT as its sole nitrogen source. TNT degradation was observed between pH 4.0-8.0 and 4-43°C; however, the most efficient degradation was at pH 6.0-7.0 and 30°C. © 2013 Elsevier Ltd.English2,4,6-Trinitrotoluene (TNT)Achromobacter spanius STE 11BiodegradationDinitrotoluenes (DNTs)Nitroaromatic compounds2 ,4 ,6-trinitrotoluene2 ,4-dinitrotoluene2 ,6-DinitrotolueneAchromobacterContaminated soilsDinitrotoluenesNitroaromatic compoundTnt-contaminated soilsMicrobiologyNitrogenSoil pollutionBiodegradationbacteriumbiodegradationmetabolitepHsamplingtolueneArticle10.1016/j.ibiod.2013.06.007