Bagislar, S.Ustuner, I.Cengiz, B.Soylemez, F.Akyerli, C. B.Ceylaner, S.Ceylaner, G.Acar, A.Ozcelik, T.2016-02-082016-02-0820060004-8666http://hdl.handle.net/11693/23708Background: The role of extremely skewed X-chromosome inactivation (XCI) has been questioned in the pathogenesis of recurrent spontaneous abortion (RSA) but the results obtained were conflicting. Aims: We therefore investigated the XCI patterns in peripheral blood DNA obtained from 80 patients who had RSA and 160 age-matched controls. Methods: Pregnancy history, age, karyotype, and disease information was collected from all subjects. The methylation status of a highly polymorphic cytosine-adenine-guanine repeat in the androgen-receptor (AR) gene was determined by use of methylation-sensitive restriction enzyme HpaII and polymerase chain reaction. Results: Skewed XCI (> 8 5% skewing) was observed in 13 of the 62 patients informative for the AR polymorphism (20.9%), and eight of the 124 informative controls (6.4%) (P = 0.0069; χ 2 test). More importantly, extremely skewed XCI, defined as > 90% inactivation of one allele, was present in 11 (17.7%) patients, and in only two controls (P = 0.0002; χ 2 test). Conclusions: These results support the interpretation that disturbances in XCI mosaicism may be involved in the pathogenesis of RSA.EnglishAndrogen receptorMosaicismMutationRecurrent abortionX-chromosome inactivationAndrogen receptorDNAAdultAlleleArticleControlled studyFemaleGene mutationGenetic analysisGenetic polymorphismGenetic susceptibilityHumanKaryotypeMajor clinical studyPathogenesisPolymerase chain reactionPriority journalProtein methylationReceptor geneRecurrent abortionRisk factorSex chromosome mosaicismSpontaneous abortionStatistical analysisTrinucleotide repeatX chromosome inactivationAbortion, HabitualAllelesDNADNA MethylationHumansMosaicismPolymorphism, GeneticPregnancyReceptors, AndrogenX Chromosome InactivationArticle10.1111/j.1479-828X.2006.00622.x