Ozdemir, A.Gursacli, R. T.Tekinay, T.2016-02-082016-02-0820140163-4984http://hdl.handle.net/11693/26627The present study characterizes the effects of the boric acid binding on calf thymus DNA (ct-DNA) by spectroscopic and calorimetric methods. UV-Vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and Fourier transform infrared (FT-IR) spectroscopy were employed to characterize binding properties. Changes in the secondary structure of ct-DNA were determined by CD spectroscopy. Sizes and morphologies of boric acid-DNA complexes were determined by transmission electron microscopy (TEM). The kinetics of boric acid binding to calf thymus DNA (ct-DNA) was investigated by isothermal titration calorimetry (ITC). ITC results revealed that boric acid exhibits a moderate affinity to ct-DNA with a binding constant (K a) of 9.54x104 M -1. FT-IR results revealed that boric acid binds to the deoxyribose sugar of DNA without disrupting the B-conformation at tested concentrations. © 2014 Springer Science+Business Media.EnglishBinding modeBoric acidCalf thymus DNAFT-IRSpectroscopyDNADNADNA bindingDNA denaturationDNA structureBovinaeAnimalsBinding SitesBoric AcidsCalorimetryCattleDeoxyriboseDNAArticle10.1007/s12011-014-9924-8