Kavak, E.Najafov, A.Ozturk, N.Seker, T.Cavusoglu, K.Aslan, T.Duru, A. D.Saygili, T.Hoxhaj, G.Hiz, M. C.Unal, D. O.Birgül-Iyison, N.Ozturk, M.Koman, A.2016-02-082016-02-0820100898-6568http://hdl.handle.net/11693/22189The Wnt signaling pathway is involved in many differentiation events during embryonic development and can lead to tumor formation after aberrant activation of its components. β-catenin, a cytoplasmic component, plays a major role in the transduction of canonical Wnt signaling. The aim of this study was to identify novel genes that are regulated by active β-catenin/TCF signaling in hepatocellular carcinoma-derived Huh7 cells with high (transfected) and low β-catenin/TCF activities. High TCF activity Huh7 cells led to earlier and larger tumor formation when xenografted into nude mice. SAGE (Serial Analysis of Gene Expression), genome-wide microarray and in silico promoter analysis were performed in parallel, to compare gene expression between low and high β-catenin/TCF activity clones, and also those that had been rescued from the xenograft tumors. SAGE and genome-wide microarray data were compared and contrasted. BRI3 and HSF2 were identified as novel targets of Wnt/β-catenin signaling after combined analysis and confirming experiments including qRT-PCR, ChIP, luciferase assay and lithium treatment. © 2010 Elsevier Inc.EnglishBRI3HCCHSF2Wnt/TCF4/β-catenin targetsXenograftArticle10.1016/j.cellsig.2010.05.021