Enhanced polymer capture speed and extended translocation time in pressure-solvation traps

Date
2018
Editor(s)
Advisor
Supervisor
Co-Advisor
Co-Supervisor
Instructor
Source Title
Physical Review E
Print ISSN
2470-0045
Electronic ISSN
Publisher
American Physical Society
Volume
97
Issue
6
Pages
062406-1 - 062406-13
Language
English
Journal Title
Journal ISSN
Volume Title
Series
Abstract

The efficiency of nanopore-based biosequencing techniques requires fast anionic polymer capture by like-charged pores followed by a prolonged translocation process. We show that this condition can be achieved by setting a pressure-solvation trap. Polyvalent cation addition to the KCl solution triggers the like-charge polymer-pore attraction. The attraction speeds-up the pressure-driven polymer capture but also traps the molecule at the pore exit, reducing the polymer capture time and extending the polymer escape time by several orders of magnitude. By direct comparison with translocation experiments [D. P. Hoogerheide, ACS Nano 8, 7384 (2014)1936-085110.1021/nn5025829], we characterize as well the electrohydrodynamics of polymers transport in pressure-voltage traps. We derive scaling laws that can accurately reproduce the pressure dependence of the experimentally measured polymer translocation velocity and time. We also find that during polymer capture, the electrostatic barrier on the translocating molecule slows down the liquid flow. This prediction identifies the streaming current measurement as a potential way to probe electrostatic polymer-pore interactions.

Course
Other identifiers
Book Title
Keywords
Citation
Published Version (Please cite this version)