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      • Faculty of Science
      • Department of Molecular Biology and Genetics
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      Differential p21 expression after ionizing and UVC radiation in EBV-transformed lymphoblastoid cells

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      Author(s)
      Moyret-Lalle, C.
      Lalle P.
      Pedeux, R.
      Guillot, C.
      Martel, S.
      Magaud J.-P.
      Puisieux, A.
      Ozturk, M.
      Date
      2000
      Source Title
      Electronic Journal of Oncology
      Print ISSN
      12928933
      Issue
      2
      Pages
      1 - 10
      Language
      English
      Type
      Article
      Item Usage Stats
      150
      views
      22
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      Abstract
      Responses to DNA-damaging agents appear to be coordinated by p53 through transcriptional activation of critical target genes. Among them, p21WAF1 encodes a protein preventing cells from entering S phase. It is not clear whether p53-mediated response varies depending on the type of DNA damage. Here, we have decided to compare the p53-mediated response of EBV-transformed lymphoblasts to ionizing radiation and UVC irradiation. We have shown that these cells respond to ionizing radiation by a cell cycle arrest as expected. Surprisingly they failed to do so after UVC treatment. Accordingly there was no significant induction of p21 protein in UVC exposed cells despite p53 accumulation. Using isogenic EBV-transformed lymphoblastoid cells expressing E6 protein of HPV18, we have demonstrated that there was no evidence of p53-dependent cell cycle arrest after UVC irradiation. These observations suggest that the p53-mediated response to UVC, in contrast to ionizing radiation, was compromised in EBV-transformed cells and might be cell type-dependent.
      Keywords
      protein E6
      protein MDM2
      protein p21
      protein p53
      article
      cell cycle
      cell cycle S phase
      cell growth
      cell transformation
      cellular distribution
      controlled study
      DNA damage
      Epstein Barr virus
      gene targeting
      genetic transcription
      growth inhibition
      human
      human cell
      ionizing radiation
      lymphoblastoid cell
      protein expression
      protein function
      protein induction
      protein localization
      ultraviolet C radiation
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      http://hdl.handle.net/11693/24939
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      • Department of Molecular Biology and Genetics 542
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