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dc.contributor.authorGulseren, G.en_US
dc.contributor.authorYasa, I. C.en_US
dc.contributor.authorUstahuseyin, O.en_US
dc.contributor.authorTekin, E. D.en_US
dc.contributor.authorTekinay, A. B.en_US
dc.contributor.authorGuler, M. O.en_US
dc.date.accessioned2016-02-08T10:15:12Z
dc.date.available2016-02-08T10:15:12Z
dc.date.issued2015en_US
dc.identifier.issn1525-7797
dc.identifier.urihttp://hdl.handle.net/11693/23525
dc.description.abstractRecognition of molecules and regulation of extracellular matrix synthesis are some of the functions of enzymes in addition to their catalytic activity. While a diverse array of enzyme-like materials have been developed, these efforts have largely been confined to the imitation of the chemical structure and catalytic activity of the enzymes, and it is unclear whether enzyme-mimetic molecules can also be used to replicate the matrix-regulatory roles ordinarily performed by natural enzymes. Self-assembled peptide nanofibers can provide multifunctional enzyme-mimetic properties, as the active sequences of the target enzymes can be directly incorporated into the peptides. Here, we report enhanced bone regeneration efficiency through peptide nanofibers carrying both catalytic and matrix-regulatory functions of alkaline phosphatase, a versatile enzyme that plays a critical role in bone formation by regulating phosphate homeostasis and calcifiable bone matrix formation. Histidine presenting peptide nanostructures were developed to function as phosphatases. These molecules are able to catalyze phosphate hydrolysis and serve as bone-like nodule inducing scaffolds. Alkaline phosphatase-like peptide nanofibers enabled osteogenesis for both osteoblast-like and mesenchymal cell lines.en_US
dc.language.isoEnglishen_US
dc.source.titleBiomacromoleculesen_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/acs.biomac.5b00593en_US
dc.subjectAlkalinityen_US
dc.subjectAmino acidsen_US
dc.subjectBiomimeticsen_US
dc.subjectBoneen_US
dc.subjectCatalyst activityen_US
dc.subjectCell cultureen_US
dc.subjectEnzymesen_US
dc.subjectMoleculesen_US
dc.subjectNanofibersen_US
dc.subjectPeptidesen_US
dc.subjectSynthesis (chemical)en_US
dc.subjectActive sequencesen_US
dc.subjectALkaline phosphataseen_US
dc.subjectBone regenerationen_US
dc.subjectExtracellular matrix synthesisen_US
dc.subjectMesenchymal cellsen_US
dc.subjectOsteogenic differentiationen_US
dc.subjectRegulatory functionsen_US
dc.subjectSelf-assembled peptidesen_US
dc.subjectPhosphatasesen_US
dc.subjectAlkaline phosphataseen_US
dc.subjectHistidineen_US
dc.subjectNanofiberen_US
dc.subjectNanomaterialen_US
dc.subjectArticleen_US
dc.subjectBone developmenten_US
dc.subjectBone matrixen_US
dc.subjectBone regenerationen_US
dc.subjectCell differentiationen_US
dc.subjectControlled studyen_US
dc.subjectHydrolysisen_US
dc.subjectMesenchyme cellen_US
dc.subjectOssificationen_US
dc.subjectOsteoblasten_US
dc.subjectPhosphate metabolismen_US
dc.subjectPriority journalen_US
dc.titleAlkaline phosphatase-mimicking peptide nanofibers for osteogenic differentiationen_US
dc.typeArticleen_US
dc.departmentInstitute of Materials Science and Nanotechnologyen_US
dc.departmentNanotechnology Research Centeren_US
dc.citation.spage2198en_US
dc.citation.epage2208en_US
dc.citation.volumeNumber16en_US
dc.citation.issueNumber7en_US
dc.identifier.doi10.1021/acs.biomac.5b00593en_US
dc.publisherAmerican Chemical Societyen_US


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