Plasmacytoid dendritic cell response to CpG ODN correlates with CXCL16 expression and is inhibited by ox-LDL
Please cite this item using this persistent URLhttp://hdl.handle.net/11693/21158
Mediators of Inflammation
- Research Paper 
Hindawi Publishing Corporation
Structurally distinct classes of synthetic CpG oligonucleotides (ODN) differentially activate human immune cells. K-type ODN trigger plasmacytoid dendritic cells (pDCs) to differentiate and produce TNFα. In contrast, D-type ODN stimulate large amounts of IFNα secretion from pDCs. The cell-surface receptor CXCL16 was previously shown to influence the nature and specificity of CpG ODN-induced immune activation. Here, we evaluated the expression and function of CXCL16 on pDC from healthy volunteers. We report that increased CXCL16 expression correlated with enhanced in vitro response exclusively to D-type CpG ODN. Conversely, enzymatic digestion of the receptor resulted in a decrease in IFNα production. Moreover, ox-LDL presence significantly inhibited D-ODN mediated IFNα production by pDCs. Coculture of enriched pDCs with the CXCR6 expressing Jurkat T cells decreased the activation threshold of these cells responding to D-ODN, suggesting that CXCL16/CXCR6 interaction may play an important role in modifying the response of pDCs to environmental danger signals. © 2013 Mayda Gursel et al.
Showing items related by title, author, creator and subject.
CD8 lineage-specific regulation of interleukin-7 receptor expression by the transcriptional repressor Gfi1 Ligons, D.L.; Tuncer, C.; Linowes, B.A.; Akcay I.M.; Kurtulus, S.; Deniz, E.; Arslan, B.A.; Cevik, S.I.; Keller H.R.; Luckey, M.A.; Feigenbaum L.; Möröy, T.; Ersahin, T.; Atalay, R.; Erman, B.; Park J.-H. (2012)Interleukin-7 receptor α (IL-7Rα) is essential for T cell survival and differentiation. Glucocorticoids are potent enhancers of IL-7Rα expression with diverse roles in T cell biology. Here we identify the transcriptional ...
Macrophage mal1 deficiency suppresses atherosclerosis in low-density lipoprotein receptor-null mice by activating peroxisome proliferator-activated receptor-γ-regulated genes Babaev V.R.; Runner, R.P.; Fan, D.; Ding L.; Zhang, Y.; Tao H.; Erbay, E.; Görgün, C.Z.; Fazio, S.; Hotamisligil G.S.; Linton, M.F. (2011)Objective- The adipocyte/macrophage fatty acid-binding proteins aP2 (FABP4) and Mal1 (FABP5) are intracellular lipid chaperones that modulate systemic glucose metabolism, insulin sensitivity, and atherosclerosis. Combined ...
Chitosan polysaccharide suppress toll like receptor dependent immune response [Çitosan polisakkaridi toll benzeri reseptöre bağlı bağışıklık yanıtını baskılar] Tincer G.; Bayyurt, B.; Arıca, Y.M.; Gürsel İ. (Turkish Society of Immunology, 2015)Objectives: Chitosan is a widely used vaccine or anti-cancer delivery vehicle. In this study, we investigated the immunomodulatory effect of chitosan/pIC nanocomplexes on mouse immune cells. Materials and methods: Proliferative ...