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dc.contributor.authorBitirim V.C.en_US
dc.contributor.authorKucukayan-Dogu G.en_US
dc.contributor.authorBengu, E.en_US
dc.contributor.authorAkcali, K.C.en_US
dc.date.accessioned2016-02-08T09:37:57Z
dc.date.available2016-02-08T09:37:57Z
dc.date.issued2013en_US
dc.identifier.issn0928-4931
dc.identifier.urihttp://hdl.handle.net/11693/20916
dc.description.abstractWe investigated the cellular adhesive features of mesenchymal stem cells (MSC) on non-coated and collagen coated patterned and vertically aligned carbon nanotube (CNT) structures mimicking the natural extra cellular matrix (ECM). Patterning was achieved using the elasto-capillary induced by water treatment on the CNT arrays. After confirmation with specific markers both at transcript and protein levels, MSCs from different passages were seeded on either collagen coated or non-coated patterned CNTs. Adhesion and growth of MSCs on the patterned CNT arrays were examined using scanning electron microscopy image analysis and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) assays. The highest MSC count was observed on the non-coated patterned CNTs at passage zero, while decreasing numbers of MSCs were found at the later passages. Similarly, MTT assay results also revealed a decrease in the viability of the MSCs for the later passages. Overall, the cell count and viability experiments indicated that MSCs were able to better attach to non-coated patterned CNTs compared to those coated with collagen. Therefore, the patterned CNT surfaces can be potentially used as a scaffold mimicking the ECM environment for MSC growth which presents an alternative approach to MSC-based transplantation therapy applications. © 2013 Elsevier B.V.en_US
dc.language.isoEnglishen_US
dc.source.titleMaterials Science and Engineering Cen_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.msec.2013.03.044en_US
dc.subjectVertically aligned carbon nanotube Stem cell Extra cellular matrix Viability Patterning Collagenen_US
dc.subjectAlternative approachen_US
dc.subjectExtracellular matricesen_US
dc.subjectMesenchymal stem cellen_US
dc.subjectProtein levelen_US
dc.subjectScanning electron microscopy imageen_US
dc.subjectThree-dimensional scaffoldsen_US
dc.subjectTransplantation therapyen_US
dc.subjectVertically aligned carbon nanotubeen_US
dc.subjectAssaysen_US
dc.subjectCarbon nanotubesen_US
dc.subjectCollagenen_US
dc.subjectFlowchartingen_US
dc.subjectScaffolds (biology)en_US
dc.subjectScanning electron microscopyen_US
dc.subjectStem cellsen_US
dc.subjectCell cultureen_US
dc.subjectcarbon nanotubeen_US
dc.subjectprimer DNAen_US
dc.subjecttissue scaffolden_US
dc.subjectanimalen_US
dc.subjectarticleen_US
dc.subjectcell proliferationen_US
dc.subjectcytologyen_US
dc.subjectmesenchymal stroma cellen_US
dc.subjectnucleotide sequenceen_US
dc.subjectpolymerase chain reactionen_US
dc.subjectraten_US
dc.subjectscanning electron microscopyen_US
dc.subjectSprague Dawley raten_US
dc.subjectWestern blottingen_US
dc.subjectcytologyen_US
dc.subjectmesenchymal stroma cellen_US
dc.subjecttissue scaffolden_US
dc.subjectAnimalsen_US
dc.subjectBase Sequenceen_US
dc.subjectBlotting, Westernen_US
dc.subjectCell Proliferationen_US
dc.subjectDNA Primersen_US
dc.subjectMesenchymal Stromal Cellsen_US
dc.subjectMicroscopy, Electron, Scanningen_US
dc.subjectNanotubes, Carbonen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectRatsen_US
dc.subjectRats, Sprague-Dawleyen_US
dc.subjectTissue Scaffoldsen_US
dc.subjectAnimalsen_US
dc.subjectBase Sequenceen_US
dc.subjectBlotting, Westernen_US
dc.subjectCell Proliferationen_US
dc.subjectDNA Primersen_US
dc.subjectMesenchymal Stromal Cellsen_US
dc.subjectMicroscopy, Electron, Scanningen_US
dc.subjectNanotubes, Carbonen_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectRatsen_US
dc.subjectRats, Sprague-Dawleyen_US
dc.subjectTissue Scaffoldsen_US
dc.titlePatterned carbon nanotubes as a new three-dimensional scaffold for mesenchymal stem cellsen_US
dc.typeArticleen_US
dc.departmentDepartment of Molecular Biology and Genetics
dc.departmentUNAM - Institute of Materials Science and Nanotechnology
dc.departmentDepartment of Chemistry
dc.departmentGenetics and Biotechnology Research Center (BilGen)
dc.citation.spage3054en_US
dc.citation.epage3060en_US
dc.citation.volumeNumber33en_US
dc.citation.issueNumber5en_US
dc.identifier.doi10.1016/j.msec.2013.03.044en_US


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