Synthesis and characterization of water dispersible conjugated polymer nanoparticles
Author
İbrahimova, Vusala
Advisor
Tuncel, Dönüş
Date
2011Publisher
Bilkent University
Language
English
Type
ThesisItem Usage Stats
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Abstract
In this study, novel water dispersible conjugated polymer nanoparticles having
various potential applications in the areas including biomedicine and photonics have
been synthesized from blue, green and yellow light emitting conjugated polymers.
Their sizes, morphology, surface charges and optical properties have been determined
using various techniques. Cell viability of nanoparticles was tested in mesenchymal
stem cells.
For the synthesis of nanoparticles, first the following polymers carrying a number of
different functional groups and based on derivatives of fluorene and benzothiodiazole
monomers are designed and synthesized using the Suzuki coupling reactions:
Poly[(9,9-bis{propeny}fluorenyl-2,7-diyl)-co-(9,9-dihexyl-9H-fluorene)] (P1),
poly[(9,9-bis{carboxymethylsulfonyl-propyl}fluorenyl-2,7-diyl)-co-(9,9-dihexyl-9Hfluorene)
(P2), poly[(9,9- bis{propeny}fluorenyl-2,7-diyl))-co-(1,4-benzo-{2,1,3}-
thiodiazole)] (P3), poly[(9,9- bis{carboxymethylsulfonyl-propyl}fluorenyl-2,7-diyl)-
co(1,4-benzo-{2,1,3}-thiodiazole)] (P4), poly[(9,9-bis{3-bromopropyl}fluorenyl-
2,7-diyl)-co-(1,4-benzo-{2,1,3}-thiodiazole)] (P5), poly[(9,9-bis{3-
azidopropyl}fluorenyl-2,7-diyl)-co-(benzothiadiazole)] (P6). Polymers were characterized by using spectroscopic techniques such as 1H-NMR,
FT-IR, UV-Vis, Fluorescence spectrophotometer and Gel Permeation
Chromatography (GPC).
Conjugated polymers carry functional groups on their side chains, such as azide and
allyl groups that can be cross-linkable using UV light to form shape-persistent, stable
nanoparticles. Nanoparticles were characterized by various techniques before and
after UV-treatment. Their sizes and morphologies were determined by using dynamic
light scattering measurements (DLS) and imaging techniques such as scanning
electron microscopy (SEM) and atomic force microscopy (AFM). For optical
characterization UV-vis, fluorescent spectroscopies and FT-IR were used.
CNPs affect on cells shows their nontoxic and biocompatible properties which give
opportunity to use them in cell imaging.