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dc.contributor.advisorAkçalı, K. Can
dc.contributor.authorÖztaş, Emin
dc.date.accessioned2016-01-08T18:21:16Z
dc.date.available2016-01-08T18:21:16Z
dc.date.issued2011
dc.identifier.urihttp://hdl.handle.net/11693/15599
dc.descriptionAnkara : The Department of Molecular Biology and Genetics and the Institute of Engineering and Science of Bilkent University, 2011.en_US
dc.descriptionThesis (Ph. D.) -- Bilkent University, 2011.en_US
dc.descriptionIncludes bibliographical references leaves 85-91.en_US
dc.description.abstractHepatocellular carcinoma (HCC) is the sixth common cancer in the world. Because of the late diagnosis of the disease, survival rates are still poor in the HCC patients. Surveillance strategies have to be developed in populations with high risk groups having premalignant diseases for HCC, such as liver cirrhosis. The usage of serum and histology-based biomarkers assists health professionals to evaluate the patients. Despite of the advances in diagnostic methods, there is still a need to develop novel biomarkers for early detection of HCC. Therefore, we aimed to develop new biomarkers with higher sensitivity and specificity for HCC to improve the surveillance of the patients. Using an apoptotic HCC cell line, HUH7, and SIP1 proteins, we generated novel monoclonal antibodies (mAbs). 6D5, 1C6 and 6E5 hybridoma clones were chosen for characterization studies because of their strong reactivity in cell-ELISA assays. We found differential reactivity pattern for those novel mAbs in a panel of human sections consisting of tumors, benign liver diseases, normal tissues and a variety of cell lines. Using proteomics methods, we identified candidate target proteins for the 6D5 mAb. Better characterization of these target proteins will provide a better understanding of the molecular pathways in the HCC and aid in the research for developing newer therapeutic agents. In conclusion, our candidate biomarker mAbs can be used in the early diagnosis of HCC as well as in drug development studies.en_US
dc.description.statementofresponsibilityÖztaş, Eminen_US
dc.format.extentxvi, 92 leaves, illustrationsen_US
dc.language.isoEnglishen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject.lccWI735 .O98 2011en_US
dc.subject.lcshCarcinoma, Hepatocellular.en_US
dc.subject.lcshLiver cancer.en_US
dc.subject.lcshCarcinogenesis.en_US
dc.subject.lcshMonoclonal antibodies.en_US
dc.subject.lcshAntibodies, Monoclonal.en_US
dc.subject.lcshAntibodies--genetics.en_US
dc.titleCharacterization of novel monoclonal antibodies that target proteins differentially expressed in hepatocellular carcinoma : a proteomics approachen_US
dc.typeThesisen_US
dc.departmentDepartment of Molecular Biology and Geneticsen_US
dc.publisherBilkent Universityen_US
dc.description.degreePh.D.en_US


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