Investigation of the effects of nicotine and levamisole on SW620 colon adenocarcinoma cells using a customized r-routine for automated microarray analysis
Author
Üçal, Muammer
Advisor
Konu, Özlen
Date
2010Publisher
Bilkent University
Language
English
Type
ThesisItem Usage Stats
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Abstract
Nicotine, the addictive component of tobacco, shows proliferative and antiapoptotic
activity in cancer cells. Levamisole, an antihelmintic, on the other hand, has been tested
as an additive chemotherapeutic agent and in treatment of nephrotic syndrome. Nicotine
and levamisole are both agonists of nicotinic acetylcholine receptors; effects of these
two agents have not been studied in colon cancer transcriptome. In this study, nicotine
and levamisole exposed SW620 colon cancer cells, at a dose of 1 μM for 7 days, were
studied with respect to changes in expression using microarrays. For data analysis, a
custom R-routine which makes extensive use of open source R-BioConductor Project
and associated packages has been written; and it is composed of three modules:
QualCont module performs quality controls supported with several visual